Mouse Liver Specific Uptake of Iodinated Growth Hormones: Evidence for The Presence of Somatogenic and Lactogenic Sites

Summary

The distribution of human growth hormone labelled with ¹²⁵I (¹²⁵I-hGH) was studied in normal adult female and male mice. The radioactivity was basically concentrated by the liver and kidney reading a maximum 15 minutes after the labelled hormone injection. Only the liver showed a significant reduction of radioactivity uptake when ¹²⁵I-hGH was injected together with an excess of unlabelled hormone. This reduction was dose-dependent and the amount of unlabelled hormone that prevented 50% of the liver uptake (ED₅₀) of ¹²⁵I-hGH was close to 3 μg for both female and male mice. Similar results were obtained in studies where bovine growth hormone labelled with ¹²⁵I (¹²⁵I-hGH) was injected, except that the maximum uptake value was significantly lower than that observed when ¹²⁵I-hGH was used. This observation could be attributed to the difference that exists between the biological properties of both hormones since hGH has growth-promoting and lactogenic effects in rodents, whereas bGH exhibits exclusively somatotropic activity.

In order to examine the nature of the radioactive material which localized in the liver soluble extracts were prepared using Triton X-100 and analyzed on Sepharose CL-6B. The majority of the radioactivity appeared as an homogeneous peak with K_D = 0.31 which could be attributed to a molecular species of Stokes radius of approximately 64Å. This magnitude is consistent with the effective molecular size reported for various hormone-receptor complexes.

Key-Words:

Human Growth Hormone - Bovine Growth Hormone - Mouse Liver - Somatogenic and Lactogenic Sites