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Horm Metab Res 1982; 14(9): 475-479
DOI: 10.1055/s-2007-1019051
© Georg Thieme Verlag, Stuttgart · New York

Inhibition of Adenylate Cyclase Activity in Human Fat Cells by α-Adrenergic Agonists

Elizabeth E. Wright, Evan R. Simpson
  • Cecil H. and Ida Green Center for Reproductive Biology Sciences and The Departments of Obstetrics and Gynecology and Biochemistry, The University of Texas Southwestern Medical School, Dallas, Texas, U.S.A.
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Publication History

1981

1981

Publication Date:
14 March 2008 (online)

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Summary

The effects of the α1-adrenergic agonist methoxamine and the α2-adrenergic agonist clonidine on isoproterenol stimulated adenylate cyclase activity were examined in plasma membranes prepared from female human subcutaneous adipose tissue. It was found that in the presence of 10 μM GTP and 100 mM NaCl increasing concentrations of both agonists inhibited basal and isoproterenol-stimulated adenylate cyclase activity. The inhibitory action of 5 × 10-7 M clonidine could not be overcome by increasing concentrations of isoproterenol. These results suggest both α1- and α2-adrenergic agonists inhibit β-agonist-stimulated adenylate cyclase activity in human adipose tissue.

Key-Words:

Adipose Tissue - Adenylate Cyclase - α-Adrenergic Agonists