Further Characterization of Human Basic-Somatomedin: Comparison with Insulin-Like Growth Factors I and II

Summary

Our basic-somatomedin (SM) was further compared with insulin-like growth factors (IGF) I and II. Basic-SM and IGF revealed similar sulfation factor (SF) activity in cartilage, insulin-like activity (ILA) in adipocytes, and receptor binding activity to adipocytes and placental cell membranes. IGF-II revealed less SF activity but more ILA than basic-SM. Comparison of SM and insulin in terms of ILA and binding activity to adipocytes suggested that adipocytes have separate insulin and SM receptors and that the ILA of SM is mediated through the SM receptors. These studies also suggest that the receptors for acidic-neutral group of SM mediate the ILA of SM, whereas the growth promoting effects of SM are mediated via receptors for the basic group of SM.

Key-Words:

Human Basic-Somatomedin (SM) - Insulin-Like Growth Factors (IGF) - Insulin-Like Activity (ILA) - Adipocytes - Placenta