Abstract
Immune changes following 2 h of intensive cycling with or without rest intervals were measured in trained cyclists (n = 12) who functioned as their own controls during two test sessions that were separated by two weeks. Subjects cycled for 2.0 h at ∼ 64 % Wattsmax continuously (C) or with 3-min rest intervals (R) interspersed every 10 min (2.6 h total time), with the order of the sessions randomized. Blood samples were collected 30-min pre-exercise, and immediately and 1-h postexercise, and assayed for blood leukocyte subset counts, plasma IL-6, IL-10, IL-1ra, IL-8, PHA-induced lymphocyte proliferation, and natural killer cell activity (NKCA). Significant time effects were measured for all immune measures, but no significant differences in the pattern of change were found between C and R exercise trials. In conclusion, immune changes induced by 2 h of intense and prolonged exercise paralleled those measured when athletes rested 3 min every 10 min of exercise.
Key words
exercise - cytokines - lymphocytes - natural killer cell activity
References
-
1
Bishop N C, Blannin A K, Robson P J, Walsh N P, Gleeson M.
The effects of carbohydrate supplementation on immune responses to a soccer-specific exercise protocol.
J Sports Sci.
1999;
17
787-796
-
2
Chang L, Gusewitch G A, Chritton D BW, Folz J C, Lebeck L K, Nehlsen-Cannarella S L.
Rapid flow cytometric assay for the assessment of natural killer activity.
J Immunol Methods.
1993;
166
45-54
-
3
Goldman H I, Becklake M R.
Respiratory function tests.
Am Rev Tuberc Pulm Dis.
1959;
79
457-467
-
4
Gray A B, Telford R D, Collins M, Weidemann M J.
The response of leukocyte subsets and plasma hormones to interval exercise.
Med Sci Sports Exerc.
1993;
25
1252-1258
-
5
Green K J, Croaker S J, Rowbottom D G.
Carbohydrate supplementation and exercise-induced changes in T-lymphocyte function.
J Appl Physiol.
2003;
95
1216-1223
-
6
Henson D A, Nieman D C, Nehlsen-Cannarella S L, Fagoaga O R, Shannon M, Bolton M R, Davis J M, Gaffney C T, Kelln W J, Austin M D, Hjertman J ME, Schilling B K.
Influence of carbohydrate on cytokine and phagocytic responses to 2 h of rowing.
Med Sci Sports Exerc.
2000;
32
1384-1389
-
7
Koch A J, Potteiger J A, Chan M A, Benedict S H, Frey B B.
Minimal influence of carbohydrate ingestion on the immune response following acute resistance exercise.
Int J Sport Nutr Exerc Metab.
2001;
11
149-161
-
8
MacKinnon L T, Jenkins D G.
Decreased salivary immunoglobulins after intense interval exercise before and after training.
Med Sci Sports Exerc.
1993;
25
678-683
-
9
Mayhew D L, Thyfault J P, Koch A J.
Rest-interval length affects leukocyte levels during heavy resistance exercise.
J Strength Cond Res.
2005;
19
16-22
-
10
Nehlsen-Cannarella S L, Fagoaga O R, Nieman D C, Henson D A, Butterworth D E, Bailey E, Warren B J, Davis J M.
Carbohydrate and the cytokine response to 2.5 hours of running.
J Appl Physiol.
1997;
82
1662-1667
-
11
Nieman D C.
Immune response to heavy exertion.
J Appl Physiol.
1997;
82
1385-1394
-
12
Nieman D C, Bishop N C.
Nutritional strategies to counter stress to the immune system in athletes: Specific reference to soccer.
J Sports Sci.
2006;
24
763-772
-
13
Nieman D C, Davis J M, Brown V A, Henson D A, Dumke C L, Utter A C, Vinci D M, Downs M F, Smith J C, Carson J, Brown A, McAnulty S R, McAnulty L S.
Influence of carbohydrate ingestion on immune changes following 2 h of intensive resistance training.
J Appl Physiol.
2004;
96
1292-1298
-
14
Nieman D C, Davis J M, Henson D A, Gross S J, Dumke C L, Utter A C, Vinci D M, Carson J A, Brown A, McAnulty S R, McAnulty L S, Triplett N T.
Skeletal muscle cytokine mRNA and plasma cytokine changes after 2.5-h cycling: influence of carbohydrate.
Med Sci Sports Exerc.
2005;
37
1283-1290
-
15
Nieman D C, Dumke C L, Henson D A, McAnulty S R, Gross S J, Lind R H.
Muscle damage is linked to cytokine changes following a 160-km race.
Brain Behav Immun.
2005;
19
398-403
-
16
Nieman D C, Kernodle M W, Henson D A, Sonnenfeld G, Morton D S.
The acute response of the immune system to tennis drills in adolescent athletes.
Res Quart Exerc Sport.
2000;
71
403-408
-
17
Nieman D C, Nehlsen-Cannarella S L, Fagoaga O R, Henson D A, Shannon M, Davis J M, Austin M D, Hisey C L, Holbeck J C, Hjertman J ME, Bolton M R, Schilling B K.
Immune response to two hours of rowing in elite female rowers.
Int J Sports Med.
1999;
20
476-481
-
18
Nieman D C, Oley K, Henson D A, Dumke C L, McAnulty S R, Davis J M, Murphy R A, Lind R H.
Ibuprofen use, endotoxemia, inflammation, and plasma cytokines during ultramarathon competition.
Brain Beh Immun.
2006;
20
578-584
-
19 Siri W E. Body composition from fluid spaces and density: analysis of methods. Brozek J, Henschel A Techniques for Measuring Body Composition. Washington, DC; National Academy of Science 1961: 223-244
-
20
Utter A C, Kang J, Nieman D C, Dumke C L, McAnulty S R.
Validation of OMNI scale of perceived exertion during prolonged cycling.
Med Sci Sports Exerc.
2006;
38
780-786
Dr. David Nieman
Appalachian State University
HLES
PO Box 32 071
28608 Boone, NC
United States
eMail: niemandc@appstate.edu
