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Int J Sports Med 2007; 28(6): 531-538
DOI: 10.1055/s-2007-964940
Nutrition

© Georg Thieme Verlag KG Stuttgart · New York

Branched-chain Amino Acids and Arginine Supplementation Attenuates Skeletal Muscle Proteolysis Induced by Moderate Exercise in Young Individuals

K. Matsumoto1 , M. Mizuno2 , T. Mizuno2 , B. Dilling-Hansen2 , A. Lahoz2 , V. Bertelsen3 , H. Münster3 , H. Jordening2 , K. Hamada1 , T. Doi1
  • 1Saga Nutraceuticals Research Institute, Otsuka Pharmaceutical Co., Ltd., Saga, Japan
  • 2Department of Anaesthesiology and Intensive Care, Ribe County Hospital, Esbjerg, Denmark
  • 3Department of Radiology, Ribe County Hospital, Esbjerg, Denmark
Further Information

Publication History

accepted after revision December 30, 2006

Publication Date:
11 May 2007 (online)

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ErratumInt J Sports Med 2007; 28(07): 630-630
DOI: 10.1055/s-2007-965512

Abstract

This study aimed at evaluating the effect of a single oral intake of branched-chain amino acids (BCAA) with Arg on skeletal muscle protein metabolism during moderate exercise in young individuals. Eight healthy volunteers (4 males and 4 females, means ± SEM, 26 ± 1 yrs, 177.8 ± 3.7 cm, 72.6 ± 3.9 kg) were studied in a randomized double-blind placebo-controlled cross-over trial. The subjects performed 3 bouts of 20-min cycling exercise (5-min break between each bout) at 126 ± 13 W corresponding to 50 % of the maximal work intensity. A single oral supplement of either a BCAA drink containing 2 g of BCAA and 0.5 g of Arg or an isocaloric placebo drink was given at 10 min of the 1st exercise bout. Both arterial and venous blood samples were simultaneously taken from the radial artery and the femoral vein, respectively. Blood flow in the femoral artery was determined using the ultrasound Doppler technique. The blood sampling and blood flow measurements were performed at rest, every 10 min during each exercise bout. Net balance of BCAA and Phe across the leg muscles were measured by the arteriovenous difference method. The BCAA ingestion resulted in increases in both the plasma BCAA concentration and BCAA uptake into the working leg. The Phe release from the leg during exercise significantly increased as compared to the basal level in the placebo trial (0.97 ± 0.28 vs. 0.23 ± 0.22 µmol/min, p < 0.05). In the BCAA trial, the cumulative Phe release from the leg during the 3rd exercise bout was significantly lower than that in the placebo trial (5.0 ± 7.4 vs. 35.9 ± 13.2 µmol/25 min, p < 0.05). These results suggest that endurance exercise at moderate intensity enhances proteolysis in working muscles, and a single oral intake of 2 g of BCAA with Arg at onset of exercise effectively suppresses exercise-induced skeletal muscle proteolysis.

Key words

amino acids - protein metabolism - moderate cycling - arteriovenous difference

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Keitaro Matsumoto

Saga Nutraceuticals Research Institute
Otsuka Pharmaceutical Co., Ltd.

Yoshinogari, Kanzaki, Saga 842 - 0195

Japan

Phone: + 81 952 52 15 22

Fax: + 81 952 53 27 27

Email: kei_matsumoto@research.otsuka.co.jp