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Int J Sports Med 2007; 28(11): 909-915
DOI: 10.1055/s-2007-964966
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Effect of NSAID on Muscle Injury and Oxidative Stress

S. McAnulty1 , L. McAnulty2 , D. Nieman1 , J. Morrow3 , C. Dumke1 , D. Henson4
  • 1Health, Leisure, and Exercise Science, Appalachian State University, Boone, NC, USA
  • 2Family and Consumer Sciences, Appalachian State University, Boone, NC, USA
  • 3Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, TN, USA
  • 4Department of Biology, Appalachian State University, Boone, NC, USA
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Publikationsverlauf

accepted after revision October 10, 2006

Publikationsdatum:
31. Mai 2007 (online)

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Abstract

Indirect markers of muscle damage and delayed onset muscle soreness were examined and correlated to changes in oxidative stress, plasma antioxidant potential, and use or nonuse of non-steroidal anti-inflammatory drugs in 60 ultra-marathoners following the Western States Endurance Run. Blood was collected prior to and immediately following the race and analyzed for muscle damage by creatine phosphokinase and oxidative stress by F2-isoprostanes, protein carbonyls, and lipid hydroperoxides and antioxidant potential by the ferric reducing ability of plasma. Subjects recorded delayed onset muscle soreness during the week following the race. Lipid hydroperoxide concentrations were unchanged, but F2-isoprostanes, protein carbonyls, ferric reducing ability of plasma, creatine phosphokinase, and delayed onset muscle soreness increased significantly postrace. Protein carbonyls were significantly higher postrace in nonsteroidal anti-inflammatory drug users versus nonusers (p < 0.05). However, there was no difference between users and non-users for all other markers. Postrace creatine phosphokinase concentrations were not correlated with oxidative stress markers but were correlated with changes in delayed onset muscle soreness. Based upon these findings, caution should be used when consuming nonsteroidal anti-inflammatory drugs during ultra distance events.

Key words

exercise - drugs - antioxidant potential - lipid oxidation - protein oxidation

References

  • 1 Ashton T, Young I S, Davison G W, Rowlands C C, McEneny J, Van B C, Jones E, Peters J R, Jackson S K. Exercise-induced endotoxemia: the effect of ascorbic acid supplementation.  Free Radic Biol Med. 2003;  35 284-291
    CrossrefPubMedSuche in Google Scholar
  • 2 Barlas P, Craig J A, Robinson J, Walsh D M, Baxter G D, Allen J M. Managing delayed-onset muscle soreness: lack of effect of selected oral systemic analgesics.  Arch Phys Med Rehabil. 2000;  81 966-972
    CrossrefPubMedSuche in Google Scholar
  • 3 Basu S, Eriksson M. Oxidative injury and survival during endotoxemia.  FEBS Lett. 1998;  438 159-160
    CrossrefPubMedSuche in Google Scholar
  • 4 Beaton L J, Allan D A, Tarnopolsky M A, Tiidus P M, Phillips S M. Contraction-induced muscle damage is unaffected by vitamin E supplementation.  Med Sci Sports Exerc. 2002;  34 798-805
    CrossrefPubMedSuche in Google Scholar
  • 5 Benzie I FF, Strain J J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay.  Anal Biochem. 1996;  239 70-76
    CrossrefPubMedSuche in Google Scholar
  • 6 Bloomer R J, Goldfarb A H, Wideman L, McKenzie M J, Consitt L A. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress.  J Strength Cond Res. 2005;  19 276-285
    CrossrefPubMedSuche in Google Scholar
  • 7 Boelsterli U A. Diclofenac-induced liver injury: a paradigm of idiosyncratic drug toxicity.  Toxicol Appl Pharmacol. 2003;  192 307-322
    CrossrefPubMedSuche in Google Scholar
  • 8 Bosenberg A T, Brock-Utne J G, Gaffin S L, Wells M T, Blake G T. Strenuous exercise causes systemic endotoxemia.  J Appl Physiol. 1988;  65 106-108
    PubMedSuche in Google Scholar
  • 9 Bourgeois J, MacDougall D, MacDonald J, Tarnopolsky M. Naproxen does not alter indices of muscle damage in resistance-exercise trained men.  Med Sci Sports Exerc. 1999;  31 4-9
    PubMedSuche in Google Scholar
  • 10 Child R B, Wilkinson D M, Fallowfield J L, Donnelly A E. Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half-marathon run.  Med Sci Sports Exerc. 1998;  30 1603-1607
    PubMedSuche in Google Scholar
  • 11 Criswell D, Powers S, Dodd S, Lawler J, Edwards W, Renshler K, Grinton S. High intensity training-induced changes in skeletal muscle antioxidant enzyme activity.  Med Sci Sports Exerc. 1993;  25 1135-1140
    PubMedSuche in Google Scholar
  • 12 Deskur E, Przywarska I, Dylewicz P, Szczesniak L, Rychlewski T, Wilk M, Wysocki H. Exercise-induced increase in hydrogen peroxide plasma levels is diminished by endurance training after myocardial infarction.  Int J Cardiol. 1998;  67 219-224
    CrossrefPubMedSuche in Google Scholar
  • 13 Dill D B, Costill D L. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration.  J Appl Physiol. 1974;  37 247-248
    CrossrefPubMedSuche in Google Scholar
  • 14 Dogra G, Ward N, Croft K D, Mori T A, Barrett P H, Herrmann S E, Irish A B, Watts G F. Oxidant stress in nephrotic syndrome: comparison of F(2)-isoprostanes and plasma antioxidant potential.  Nephrol Dial Transplant. 2001;  16 1626-1630
    PubMedSuche in Google Scholar
  • 15 Donnelly A E, McCormick K, Maughan R J, Whiting P H, Clarkson P M. Effects of a non-steroidal anti-inflammatory drug on delayed onset muscle soreness and indices of damage.  Br J Sports Med. 1988;  22 35-38
    CrossrefPubMedSuche in Google Scholar
  • 16 Goldfarb A H, Patrick S W, Bryer S, You T. Vitamin C supplementation affects oxidative-stress blood markers in response to a 30-minute run at 75 % V·O2max.  Int J Sport Nutr Exerc Metab. 2005;  15 279-290
    PubMedSuche in Google Scholar
  • 17 Goto S, Nakamura A, Radak Z, Nakamoto H, Takahashi R, Yasuda K, Sakurai Y, Ishii N. Carbonylated proteins in aging and exercise: immunoblot approaches.  Mech Ageing Dev. 1999;  107 245-253
    CrossrefPubMedSuche in Google Scholar
  • 18 Hinchcliff K W, Reinhart G A, DiSilvestro R, Reynolds A, Blostein-Fujii A, Swenson R A. Oxidant stress in sled dogs subjected to repetitive endurance exercise.  Am J Vet Res. 2000;  61 512-517
    CrossrefPubMedSuche in Google Scholar
  • 19 Inayama T, Kumagai Y, Sakane M, Saito M, Matsuda M. Plasma protein-bound sulfhydryl group oxidation in humans following a full marathon race.  Life Sci. 1996;  59 573-578
    CrossrefPubMedSuche in Google Scholar
  • 20 Ji L L. Exercise at old age: does it increase or alleviate oxidative stress?.  Ann NY Acad Sci. 2001;  928 236-247
    PubMedSuche in Google Scholar
  • 21 Koller A, Mair J, Schobersberger W, Wohlfarter T, Haid C, Mayr M, Villiger B, Frey W, Puschendorf B. Effects of prolonged strenuous endurance exercise on plasma myosin heavy chain fragments and other muscular proteins. Cycling vs. running.  J Sports Med Phys Fitness. 1998;  38 10-17
    PubMedSuche in Google Scholar
  • 22 Lee J, Goldfarb A H, Rescino M H, Hegde S, Patrick S, Apperson K. Eccentric exercise effect on blood oxidative-stress markers and delayed onset of muscle soreness.  Med Sci Sports Exerc. 2002;  34 443-448
    PubMedSuche in Google Scholar
  • 23 Leeuwenburgh C, Hansen P A, Holloszy J O, Heinecke J W. Oxidized amino acids in the urine of aging rats: potential markers for assessing oxidative stress in vivo.  Am J Physiol. 1999;  276 R128-R135
    PubMedSuche in Google Scholar
  • 24 Mastaloudis A, Morrow J D, Hopkins D W, Devaraj S, Traber M G. Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners.  Free Radic Biol Med. 2004;  36 1329-1341
    CrossrefPubMedSuche in Google Scholar
  • 25 McAnulty S R, McAnulty L, Pascoe D D, Gropper S S, Keith R E, Morrow J D, Gladden L B. Hyperthermia increases exercise-induced oxidative stress.  Int J Sports Med. 2005;  26 188-192
    Thieme ConnectPubMedSuche in Google Scholar
  • 26 McAnulty S R, McAnulty L S, Nieman D C, Morrow J D, Shooter L A, Holmes S, Heward C, Henson D A. Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise.  J Nutr Biochem. 2005;  16 530-537
    PubMedSuche in Google Scholar
  • 27 Morrow J D. The isoprostanes: their quantification as an index of oxidant stress status in vivo.  Drug Metab Rev. 2000;  32 377-385
    CrossrefPubMedSuche in Google Scholar
  • 28 Morrow J D, Roberts L J. Mass spectrometric quantification of F2-isoprostanes in biological fluids and tissues as measure of oxidant stress.  Methods Enzymol. 1999;  300 3-12
    PubMedSuche in Google Scholar
  • 29 Morrow J D, Roberts L J. The isoprostanes: unique bioactive products of lipid peroxidation.  Prog Lipid Res. 1997;  36 1-21
    PubMedSuche in Google Scholar
  • 30 Nieman D C, Dumke C I, Henson D A, McAnulty S R, McAnulty L S, Lind R H, Morrow J D. Immune and oxidative changes during and following the Western States Endurance Run.  Int J Sports Med. 2003;  24 541-547
    Thieme ConnectPubMedSuche in Google Scholar
  • 31 Nieman D C, Dumke C L, Henson D A, McAnulty S R, Gross S J, Lind R H. Muscle damage is linked to cytokine changes following a 160-km race.  Brain Behav Immun. 2005;  19 398-403
    CrossrefPubMedSuche in Google Scholar
  • 32 Nourooz-Zadeh J, Tajaddini-Sarmadi J, Ling E, Wolff S. Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma.  Bio J. 1996;  313 781-786
    PubMedSuche in Google Scholar
  • 33 Nyyssonen K, Porkkala-Sarataho E, Kaikkonen J, Salonen J T. Ascorbate and urate are the strongest determinants of plasma antioxidative capacity and serum lipid resistance to oxidation in Finnish men.  Atherosclerosis. 1997;  130 223-233
    CrossrefPubMedSuche in Google Scholar
  • 34 Saxton J M, Donnelly A E, Roper H P. Indices of free-radical mediated damage following maximum voluntary eccentric and concentric muscular work.  Eur J Appl Physiol. 1994;  68 189-193
    CrossrefPubMedSuche in Google Scholar
  • 35 Tokmakidis S P, Kokkinidis E A, Smilios I, Douda H. The effects of ibuprofen on delayed muscle soreness and muscular performance after eccentric exercise.  J Strength Cond Res. 2003;  17 53-59
    CrossrefPubMedSuche in Google Scholar
  • 36 Vider J, Lehtmaa J, Kullisaar T, Vihalemm T, Zilmer K, Kairane C, Landor A, Karu T, Zilmer M. Acute immune response in respect to exercise-induced oxidative stress.  Pathophysiology. 2001;  7 263-270
    CrossrefPubMedSuche in Google Scholar
  • 37 Warren J A, Jenkins R R, Packer L, Witt E H, Armstrong R B. Elevated muscle vitamin E does not attenuate eccentric exercise-induced muscle injury.  J Appl Physiol. 1992;  72 2168-2175
    PubMedSuche in Google Scholar

PhD Steven McAnulty

Health, Leisure, and Exercise Science
Appalachian State University

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Boone, NC 28608

USA

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eMail: mcanltysr@appstate.edu