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Planta Med 1986; 52(2): 85-87
DOI: 10.1055/s-2007-969086
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© Georg Thieme Verlag Stuttgart · New York

Tropane Alkaloids from Atropa belladonna; Part I. Capillary Gas Chromatographic Analysis

Markku Ylinen1 , Toivo Naaranlahti1 , Seppo Lapinjoki1 , Aarre Huhtikangas1 , Maija-Liisa Salonen2 , Liisa Kaarina Simola2 , Mauri Lounasmaa3
  • 1Department of Pharmaceutical Chemistry, University of Kuopio POB 6, SF-70211 Kuopio, Finland
  • 2Department of Botany, University of Helsinki, Unioninkatu 44, SF-00170 Helsinki, Finland
  • 3Department of Chemistry, Technical University of Helsinki, SF-02150 Espoo, Finland
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Publication History

1985

Publication Date:
26 February 2007 (online)

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Abstract

Atropine and scopolamine were assayed in leaf samples and cultured cells of Atropa belladonna L. by capillary gas chromatography. The samples were extracted and purified before the gas Chromatographic analysis by passage through disposable extraction columns of reversed phase type. Deviation among the analyses was 4.0% for atropine and 2.9% for scopolamine at 50 mg/l. The limit of detection was 5 mg/l for both alkaloids.