Isolation of Spinasterol and its Glucoside from Cell Suspension Cultures of Saponaria officinalis: ¹³C-NMR Spectral Data and Batch Culture Production

Abstract

Spinasterol and spinasteryl glucoside have been isolated as the exclusive sterols from cell suspension cultures of Saponaria officinalis and identified by MS, ¹H-NMR and ¹³C-NMR spectral data. This result confirms the taxonomical vicinity of this plant to Chenopodiaceae and Amaranthaceae where Δ⁷-sterols were also described to be present. With more than 44% of total sterol content, the spinasteryl glucoside content is higher than in most of the common plants. This shows a particularly active glycosylation process probably in relation with the biosynthesis of saponosides.