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Planta Med 1983; 49(12): 222-225
DOI: 10.1055/s-2007-969855
Research Articles

© Hippokrates Verlag Stuttgart

Assay Method for Antihepatotoxic Activity Using Carbon Tetrachloride Induced Cytotoxicity in Primary Cultured Hepatocytes*

Y. Kiso, M. Tohkin, H. Hikino
  • Pharmaceutical Institute, Tohoku University, Sendai, Japan
* Liver-protective drugs, Part 6. This paper is also Part 51 in the series on the validity of Oriental medicines.
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Publication History

1982

1983

Publication Date:
26 March 2007 (online)

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Abstract

Conditions were examined to devise an in vitro assay method for antihepatotoxic activity using carbon tetrachloride-induced lesion in primary cultured mouse and rat liver cells. Utilizing 24 hr-preincubated hepatocytes from rats, which give more cells and are more sensitive to the hepatotoxin than mice, a satisfactory assay procedure was established. A good correlation of results obtained by this in vitro assay method with those by in vivo assay methods was corroborated by screening of some natural products. This assay method offers many advantages: numerous samples disposed at one time at a low expense, small sample sizes required, and little variation and good reproducibility of results.

Key Word Index

Antihepatotoxic activity - Carbon tetrachloride induced cytotoxicity - Glutamic-pyruvic transaminase activity - Mouse - Primary cultured hapatocytes - Rat