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Planta Med 1983; 48(6): 67-71
DOI: 10.1055/s-2007-969888
Research Articles

© Hippokrates Verlag Stuttgart

Determination of Aconitine Alkaloids in Aconitum Roots*

H. Hikino1 , M. Murakami1 , Ch. Konno1 , H. Watanabe2
  • 1Pharmaceutical Institute, Tohoku University, Sendai, Japan
  • 2Tokyo Research Laboratories, Toyo Soda Mfg. Co., Hayakawa, Ayase, Kanagawa, Japan
* Pharmaceutical studies on Aconitum roots, Part 15. This paper also forms Part 47 in the series on the validity of the Oriental medicines.
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Publication History

1982

1983

Publication Date:
26 March 2007 (online)

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Abstract

A reliable method for extraction of the crude drug, Aconitum roots, and purification of the extract for its eventual separation and quantitation of the aconitine alkaloids by HPLC technique has been developed. The method comprises extraction of aconite powder first with ammoniacal ether and then with methanol (× 3 each), chromatography of the resultant extract over neutral alumina and elution with ethyl acetate-methanol (7:3) prior to HPLC. The method has been found to be reproducible and suitable for routine analysis of aconite and its pharmaceutical preparations.

Key Word Index

Aconitum carmichaeli - Aconitum japonicum - Ranunculaceae - Aconitines - Benzoylaconines - High-performance liquid chromatography - Quantitative determination