Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents

B. N. Meyer; N. R. Ferrigni; J. E. Putnam; L. B. Jacobsen; D. E. Nichols; J. L. McLaughlin

doi:10.1055/s-2007-971236

Planta Medica, Table of Contents

Planta Med 1982; 45(5): 31-34
DOI: 10.1055/s-2007-971236

Research Articles

Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents

B. N. Meyer¹ , N. R. Ferrigni¹ ^, ² , J. E. Putnam¹ , L. B. Jacobsen¹ , D. E. Nichols¹ , J. L. McLaughlin¹

¹Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907
²Visiting Assistant Professor from the Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela.

Abstract

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Abstract

A method, utilizing brine shrimp (Artemia salina LEACH), is proposed as a simple bioassay for natural product research. The procedure determines LC₅₀ values in µg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool.

Key Word Index

Artemia salina - Brine shrimp - Bioassay methods - Euphorbiaceae seeds - 9KB and 9PS cytotoxicities

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