Application of Radioimmunoassay to the Quantitation of Hyoscyamine in Plant Material

T. Lehtola; A. Huhtikangas; R. Virtanen

doi:10.1055/s-2007-971380

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Planta Med 1982; 45(8): 237-239
DOI: 10.1055/s-2007-971380

Research Articles

Application of Radioimmunoassay to the Quantitation of Hyoscyamine in Plant Material

T. Lehtola¹ , A. Huhtikangas² , R. Virtanen³

¹Division of Pharmacognosy, School of Pharmacy, University of Helsinki, Finland
²Department of Pharmacy, University of Kuopio, Finland
³Department of Pharmacology, Institute of Biomedicine, University of Turku, Finland

Further Information

Publication History

1981

Publication Date:
29 March 2007 (online)

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Abstract

A radioimmunoassay has been developed for the analysis of atropine (dl-hyoscyamine) from unpurified ethanolic extracts of Atropa belladonna L. The antiserum used was highly specific for d-hyoscyamine, the unnatural enantiomer. Consequently, a racemization step had to be included in the assay procedure in order to obtain the analysis of atropine. The method is rapid and sensitive, giving excellent values for accuracy, precision and correlation. There was practically no interference in the assay from other compounds present in the extracts.

Key Word Index

Atropa belladonna - Radioimmunoassay - Crossreaction - Atropine - Hyoscyamine - Racemization