Planta Medica, Inhaltsverzeichnis Planta Med 1982; 45(8): 237-239DOI: 10.1055/s-2007-971380 Research Articles © Hippokrates Verlag Stuttgart Application of Radioimmunoassay to the Quantitation of Hyoscyamine in Plant Material T. Lehtola1 , A. Huhtikangas2 , R. Virtanen3 1Division of Pharmacognosy, School of Pharmacy, University of Helsinki, Finland 2Department of Pharmacy, University of Kuopio, Finland 3Department of Pharmacology, Institute of Biomedicine, University of Turku, Finland Artikel empfehlen Abstract als PDF herunterladen Artikel einzeln kaufen Abstract A radioimmunoassay has been developed for the analysis of atropine (dl-hyoscyamine) from unpurified ethanolic extracts of Atropa belladonna L. The antiserum used was highly specific for d-hyoscyamine, the unnatural enantiomer. Consequently, a racemization step had to be included in the assay procedure in order to obtain the analysis of atropine. The method is rapid and sensitive, giving excellent values for accuracy, precision and correlation. There was practically no interference in the assay from other compounds present in the extracts. Key Word Index Atropa belladonna - Radioimmunoassay - Crossreaction - Atropine - Hyoscyamine - Racemization PDF (201 kb)
