An ionpair-HPLC-technic using a reversed-phase column was developed to screen plant extracts for cardiotonic amines and to isolate them in a preparative scale for direct MS and pharmacological investigations. By this method the positive inotropic tyramine could be isolated from Selenicereus grandiflorus (L.) BRITT et ROSE (Cactus grandiflorus).
Selenicereus grandiflorus - Cactaceae - Cactus grandiflorus - Ionpair-high-performance-liquid-chromatography - Cardiotonic plant-amines - Tyramine