Planta Med 1982; 44(4): 237-240
DOI: 10.1055/s-2007-971457
Research Articles

© Hippokrates Verlag Stuttgart

Tissue Culture of Cinchona pubescens: Effects of Media Modifications on the Growth

Th. Mulder-Krieger, R. Verpoorte, A. Baerheim Svendsen
  • Department of Pharmacognosy, State University of Leiden, Gorlaeus Laboratories, Leiden, The Netherlands
Further Information

Publication History

1981

Publication Date:
29 March 2007 (online)

Abstract

Studies were performed on the growth of callus cultures of Cinchona pubescens VAHL concerning the influence of the concentration of major and minor elements described by MURASHIGE and SKOOG [1], as well as a number of organic constituents in the medium.

After 13 weeks a maximum increase of the fresh and dry weight was observed in tissues grown on a medium containing 2,4-D[1] (1 mg/l) and BA[1] (2 mg/l) as growth factors and with the same or the half concentration of mineral salts as used by MURASHIGE and SKOOG.

1 2,4-D: 2,4-Dichlorophenoxyacetic acid, BA: Benzyladenine

1 2,4-D: 2,4-Dichlorophenoxyacetic acid, BA: Benzyladenine