Reversed-Phase High-Performance Liquid Chromatography Analysis of Apigenin and its Glucosides in Flowers of Matricaria chamomilla and Chamomile Extracts

C. Redaelli; L. Formentini; E. Santaniello

doi:10.1055/s-2007-971643

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Planta Med 1981; 42(7): 288-292
DOI: 10.1055/s-2007-971643

Research Articles

Reversed-Phase High-Performance Liquid Chromatography Analysis of Apigenin and its Glucosides in Flowers of Matricaria chamomilla and Chamomile Extracts

C. Redaelli¹ , L. Formentini¹ , E. Santaniello²

¹Research Laboratory, Bonomelli S.p.A., Dolzago
²Istituto di Chimica, Facoltà di Medicina, Università di Milano, Milano, Italy

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Publication History

Publication Date:
30 March 2007 (online)

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Abstract

Quantitative estimation of the flavones apigenin, apigenin-7-glucoside and apigenin-7-acetylglucoside in ligulate florets of Matricaria chamomilla was performed by HPLC using a reverse-phase column and eluting with acetonitrile/water, acetic acid system. These flavonoids were detected at 335 nm. Apigenin and its glucosides were not found in the tubular florets. The method was also applied to the estimation of these flavonoids in chamomile extracts.

Key Word Index

Matricaria chamomilla - Asteraceae (Compositae) Flavonoids - Apigenin - Ligulate flowers