An investigation of the Accusport® Lactate Analyser (ACC) was undertaken to assess its accuracy, linearity, reliability, and versatility. Accuracy was determined by comparing 32 blood assays on the ACC with those obtained using the Kodak Ekta-chem® E250 (E250). Linearity was determined over the range 1.2 - 18.7mmol/L. Reliability was measured at 1.7 (low) and 14.4 (high) mmol/L concentrations. Versatility involved determining the optimal sample volume, influence of haematocrit, and the effect of time delay from sampling to assay. Assays determined using the ACC confirm good agreement with the E250. The difference between the two methods compared for lactate determination does not exceed 1.1 mmol/L for concentrations up to approximately 20 mmol/L. Linearity is demonstrated by the regression equation y = 0.026 + 0.970 x, r = 0.995. Reliability is expressed by the coefficient of variation of 7 % (low) and 4.6 % (high). The optimal sample size for accurate assay was determined to be between 20 μL and 50 μL. Mean lactate value did not vary significantly between a haematocrit of 35 % and 45 %. The Accusport is accurate, linear up to at least 18.7 mmol/L, has good reliability at high and low concentrations, and is able to analyse whole blood up to 15 minutes after sample application to the reagent strip.
Key words
Lactate - blood lactate - Accusport - lactate measurement - reflectance photometry
