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Horm Metab Res 1999; 31(12): 653-656
DOI: 10.1055/s-2007-978815
Originals Clinical

© Georg Thieme Verlag Stuttgart · New York

Effect of 1α,25(OH)2-Vitamin D3 on TNFα-Mediated Apoptosis of Human Primary Osteoblast-Like Cells in Vitro

E. Pascher1 , A. Perniok2 , A. Becker1 , J. Feldkamp1
  • 1Department of Endocrinology, Heinrich-Heine University of Düsseldorf, Germany
  • 2Department of Nephrology and Rheumatology, Heinrich-Heine University of Düsseldorf, Germany
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Publication History

1999

1999

Publication Date:
20 April 2007 (online)

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Abstract

1α,25(OH)2 vitamin D3 is a hormone which potentially stimulates bone cell growth and differentiation. TNFα is one possible inductor for apoptosis; apoptosis being an important regulatoring factor for bone modelling and remodelling. We examined the influence of physiological levels (0.1 nM) 1α25(OH)2-vitamin D3TNFα-mediated apoptosis in human osteoblast-like cells. These human cells were obtained from bone fragments obtained during orthopedic operations on patients without systemic bone disease. Treatment with 1α,25(OH)2 vitamin D3 for 8 weeks resulted in a significant reduction (30%) of viable cell number compared to untreated cells. Incubation with TNFα (100 ng/ml for 4 hours) only had limited effects on the rate of apoptosis in control cells. After pretreatment with 1α25(OH) 2-vitamin D3induction of apoptosis increased up to 10% in human osteoblast-like cells. In parallel to the induction of apoptosis, 1α,25(OH)2-vitamin D3 stimulated osteocalcin and alkaline phosphatase as markers of mature osteoblasts. Our data suggest that 1α25(OH)2-vitamin D3 has a stimulatory effect on TNFα-induced apoptosis in human osteoblast-like cells as a result of 1α25(OH)2-vitamin D3-induced cell differentiation.

Key words

Osteoblasts - Apoptosis - Vitamin D3 - TNFα