Vitamin D Receptor Expression in Chicken Muscle Tissue and Cultured Myoblasts

S. B. Zanello; E. D. Collins; M. J. Marinissen; A. W. Norman; R. L. Boland

doi:10.1055/s-2007-979027

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1997; 29(5): 231-236
DOI: 10.1055/s-2007-979027

Originals Basic

Vitamin D Receptor Expression in Chicken Muscle Tissue and Cultured Myoblasts

S. B. Zanello¹ , E. D. Collins² , M. J. Marinissen¹ , A. W. Norman² , R. L. Boland¹

¹Departamento de Biología, Bioquimica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina
²Department of Biochemistry, University of California, Riverside, California, U.S.A.

Abstract

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Muscle has long been recognized as a target tissue for 1,25-dihydroxy-vitamin D₃ (1,25[OH]₂D₃). Evidence of the presence of VDR is provided here, thus supporting the existence of a receptor-mediated mechanism of action of 1,25(OH)₂D₃. Vitamin D receptor (VDR) expression is evidenced by detection of VDR-mRNA, through reverse transcription and polymerase chain reaction (RT/PCR), in chicken muscle and muscle cells (myoblasts) as well as in a variety of tissues such as intestine, kidney, heart and brain. VDR presence is also demonstrated by Southern blot of PCR products with a specific VDR-cDNA probe and by immunocytochemistry carried out on myoblasts and cardiac myocytes. Localization of VDR is mainly nuclear and more faintly detected in the cytosol.

Key words

1,25-Dihydroxy-vitamin D₃ - Muscle (Chicken) - Vitamin D Receptor - Immunostaining - PCR

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