Glyceraldehyde 3-Phosphate-Induced DNA or Protein Modifications Severely Inhibit the Protein/DNA Interaction

Marina Moro; A. Ceriello; Franceschina Mercuri; G. Tell; Lucia Pellizzari; G. Damante

doi:10.1055/s-2007-979051

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Horm Metab Res 1997; 29(7): 347-350
DOI: 10.1055/s-2007-979051

Original Basics

Glyceraldehyde 3-Phosphate-Induced DNA or Protein Modifications Severely Inhibit the Protein/DNA Interaction

Marina Moro¹ , A. Ceriello² , Franceschina Mercuri¹ , G. Tell¹ , Lucia Pellizzari¹ , G. Damante¹

¹Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Italy
²Dipartimento di Patologia e Medicina Sperimentale e Clinica, Università di Udine, Italy

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Publication History

1996

1997

Publication Date:
23 April 2007 (online)

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In this study, the effect of the reducing sugar glyceraldehyde 3-phosphate on protein/DNA interaction has been investigated. Treatment with glyceraldehyde 3-phosphate of oligonucleotides recognized by various transcription factors severely inhibits protein binding. The inhibitory effect is time and dose-dependent. Treatment with glyceraldehyde 3-phosphate of the homeodomain protein TTF-1 HD has also an inhibitory effect on the interaction with DNA, again in a time and dose-dependent manner. These “in vitro” effects could have “in vivo” counterparts and therefore contribute to molecular alterations observed either when intracellular protein are exposed to high doses of reducing sugars (i.e. in diabetes) or after a long time exposure (i.e. in G₀-arrested cells during aging).

Key words

Reducing Sugars - DNA - Homeodomain - Paired Domain - Diabetes