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Horm Metab Res 1995; 27(10): 455-460
DOI: 10.1055/s-2007-980001
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© Georg Thieme Verlag Stuttgart · New York

Effect of 1,10-Phenanthroline on DNA Binding by 1,25-(OH)2 Vitamin D Receptor

K. B. Atkins, D. C. Beitz
  • Department of Animal Science, Iowa State University, Ames, Iowa, USA
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Publication History

1994

1995

Publication Date:
23 April 2007 (online)

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Abstract

The effect of the chelator 1,10-phenanthroline on the DNA-binding of 1,25-dihydroxyvitamin D receptor was demonstrated. Increasing concentrations of the chelator, up to 3 mM, decreased receptor binding to DNA-cellulose. This effect was specific for the chelating function because the nonchelating analog, 1,7-phenanthroline, did not inhibit DNA-cellulose binding of the receptor. The cations Cd2+ and Zn2+ were able to block chelator-mediated inhibition. These cations were unable to reverse the inhibition unless 1,10-phenanthroline was removed by dialysis.

Key words

1,10-Phenanthroline - DNA - 1,25-Dihydroxyvitamin D Receptor