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Horm Metab Res 1995; 27(10): 455-460
DOI: 10.1055/s-2007-980001
DOI: 10.1055/s-2007-980001
Originals Basic
© Georg Thieme Verlag Stuttgart · New York
Effect of 1,10-Phenanthroline on DNA Binding by 1,25-(OH)2 Vitamin D Receptor
Further Information
Publication History
1994
1995
Publication Date:
23 April 2007 (online)
![](https://www.thieme-connect.de/media/hmr/199510/lookinside/thumbnails/10.1055-s-2007-980001-1.jpg)
Abstract
The effect of the chelator 1,10-phenanthroline on the DNA-binding of 1,25-dihydroxyvitamin D receptor was demonstrated. Increasing concentrations of the chelator, up to 3 mM, decreased receptor binding to DNA-cellulose. This effect was specific for the chelating function because the nonchelating analog, 1,7-phenanthroline, did not inhibit DNA-cellulose binding of the receptor. The cations Cd2+ and Zn2+ were able to block chelator-mediated inhibition. These cations were unable to reverse the inhibition unless 1,10-phenanthroline was removed by dialysis.
Key words
1,10-Phenanthroline - DNA - 1,25-Dihydroxyvitamin D Receptor