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Viszeralchirurgie 2007; 42(3): 125-133
DOI: 10.1055/s-2007-981186
Aktuelle Chirurgie

© Georg Thieme Verlag Stuttgart · New York

Tumor Proteomics - Neue Wege in der Diagnostik kolorektaler Karzinome

Cancer Proteomics - Innovative Approaches in Colorectal Cancer DiagnosticsF. G. Bader1 , 2 , J. K. Habermann1 , K. Zimmermann1 , T. Gemoll1 , C. Franke1 , H.-P. Bruch1 , G. Auer2 , U. J. Roblick1 , 2
  • 1Klinik für Chirurgie, Arbeitsgruppe Tumorbiomics, Universitätsklinikum Schleswig-Holstein, Campus Lübeck, Lübeck, Deutschland
  • 2Karolinska Biomic Center, KBC, Karolinska Institutet, Stockholm, Schweden
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Publication History

Publication Date:
27 June 2007 (online)

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Zusamenfassung

Die Frühdiagnose des kolorektalen Karzinoms respektive der zum Malignom führenden Vorstufen ist der Schlüssel zur kurativen Therapie dieser Erkrankung. Trotz Screening-Methoden werden über zwei Drittel aller kolorektalen Karzinome erst im Stadium III und IV diagnostiziert und bedingen so die schlechte Prognose. Die Proteomforschung („PROTEins expressed by a genOME”) ist ein neues Feld der Wissenschaft, in dem Zusammensetzung, Struktur, Funktion und Interaktion von Polypeptiden untersucht werden, die als potenzielle Biomarker zur Früherkennung fungieren können. Schon heute sind Proteomanalysen auf Basis der zweidimensionalen Gelelektrophorese (2-DE), der Massenspektrometrie (MALDI) und der SELDI-Technologie in der Lage, mit hoher Sensitivität und Spezifität den Tumorerkrankten vom Gesunden zu unterscheiden. Die Identifikation tumorentitätsspezifischer Markerproteine wird es zukünftig möglich machen, personalisierte Behandlungsstrategien zu entwickeln und birgt somit das Potenzial, die Überlebensraten von Patienten mit kolorektalen Karzinomen deutlich zu beeinflussen. Es ist vorhersehbar, dass in naher Zukunft die klinische Medizin die Ergebnisse der Proteom- und Genomforschung in die Routine integrieren wird. Bei diesem Transfer von „bench to bedside” wird dem Chirurgen eine tragende Rolle zukommen.

Abstract

Detection of colorectal cancer at an early stage or even before it occurs, is critical when curative treatment is intented. Despite all possibilties to screen for colorectal cancer, more than two thirds are diagnosed at advanced stages (UICC III and IV) of disease. This determines the growing demand for efficient diagnostic and prognostic markers. Proteomic research (“PROTEins expressed by a genOME”) approchaches issues like protein abundance, posttranslational polypeptide modification and protein-protein interaction as well as functional and dynamic processes within the cell and can therefore lead to biomarker discovery for early disease detection. Already now, proteomics based on 2-dimensional gel electrophoresis, masspectrometry (MALDI) and SELDI-technology, can distinguish a healty from the tumor patient with high sensitivity and specificity. The identification of tumor specific proteins will enable us to develop patient tailored treatment strategies, that have the potential to improve the survival of patients suffering from colorectal cancer. It can be foreseen that clinical medicine will integrate the results of proteome and genome research into daily routine. Within this transfer from “bench to bedside” the surgeon will play a pivotal role.

Schlüsselwörter

Proteomics - kolorektales Karzinom - Tumormarker

Key words

proteomics - colorectal cancer - biomarker

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Dr. Dr. med. U. J. Roblick

Klinik für Chirurgie · Universitätsklinikum Schleswig-Holstein · Campus Lübeck

Ratzeburgerallee 160

23538 Lübeck

Deutschland

Phone: +49/4 51/5 00 63 37

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Email: DrDr.UJRoblick@t-online.de