Planta Med 2007; 73(7): 674-678
DOI: 10.1055/s-2007-981525
Pharmacology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Immunosuppressive Lignans Isolated from Saururus chinensis

Shin-Young Park1 , Sung Hak Lee1 , Woo Hyuck Choi1 , Eun Mi Koh1 , Jee Hee Seo2 , Shi Yong Ryu2 , Young Sup Kim2 , Dae Young Kwon3 , Woo Suk Koh1
  • 1Korea Institute of Toxicology, Daejeon, Korea
  • 2Korea Research Institute of Chemical Technology, Daejeon, Korea
  • 3Korea Food Research Institute, Sungnam, Kyunggi-do, Korea
Further Information

Publication History

Received: July 5, 2006 Revised: April 11, 2007

Accepted: April 16, 2007

Publication Date:
31 May 2007 (online)

Abstract

Bioactivity-guided fractionation of Saururus chinensis (Saururaceae) using a lymphoproliferation assay led us to isolate 5 lignans (compounds 1 - 5). Compounds 1 - 5 were identified as sauchinone, (-)-saucerneol, saucerneol C, manassantin A, and manassantin B, respectively, by spectroscopic analyses. The immunosuppressive activities of the active compounds were evaluated using lymphoproliferation, mixed leukocyte response, and Th1/Th2 cytokine assays. The relative potency was in the order: manassantin A, B > (-)-saucerneol > saucerneol C > sauchinone.

Abbreviations

Con A: concanavalin A

EBSS: Earle’s balanced salt solution

ELISA: enzyme-linked immunosorbent assay

IL-2: interleukin-2

INFγ: interferon gamma

LPS: lipopolysaccharide

MLR: mixed lymphocyte response

RPMI: Roswell Park Memorial Institute

TNFα: tumor necrosis factor alpha

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Woo Suk Koh, Ph D.

Korea Institute of Toxicology

100 Jangdong

Yusung

Daejeon 305-343

Korea

Phone: +82-42-610-8091

Fax: +82-42-610-8172

Email: wskoh@kitox.re.kr