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DOI: 10.1055/s-2007-981525
© Georg Thieme Verlag KG Stuttgart · New York
Immunosuppressive Lignans Isolated from Saururus chinensis
Publication History
Received: July 5, 2006
Revised: April 11, 2007
Accepted: April 16, 2007
Publication Date:
31 May 2007 (online)
![](https://www.thieme-connect.de/media/plantamedica/200707/lookinside/thumbnails/10.1055-s-2007-981525-1.jpg)
Abstract
Bioactivity-guided fractionation of Saururus chinensis (Saururaceae) using a lymphoproliferation assay led us to isolate 5 lignans (compounds 1 - 5). Compounds 1 - 5 were identified as sauchinone, (-)-saucerneol, saucerneol C, manassantin A, and manassantin B, respectively, by spectroscopic analyses. The immunosuppressive activities of the active compounds were evaluated using lymphoproliferation, mixed leukocyte response, and Th1/Th2 cytokine assays. The relative potency was in the order: manassantin A, B > (-)-saucerneol > saucerneol C > sauchinone.
Abbreviations
Con A: concanavalin A
EBSS: Earle’s balanced salt solution
ELISA: enzyme-linked immunosorbent assay
IL-2: interleukin-2
INFγ: interferon gamma
LPS: lipopolysaccharide
MLR: mixed lymphocyte response
RPMI: Roswell Park Memorial Institute
TNFα: tumor necrosis factor alpha
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- Supporting Information .
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Woo Suk Koh, Ph D.
Korea Institute of Toxicology
100 Jangdong
Yusung
Daejeon 305-343
Korea
Phone: +82-42-610-8091
Fax: +82-42-610-8172
Email: wskoh@kitox.re.kr
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