1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, P. R. China
2Graduate School of Chinese Academy of Sciences, Beijing, P. R. China
3Guangxi Institue of Chinese Medicine & Pharmaceutical Science, Nanning, P. R. China
Two new quaternary isoquinoline alkaloids, saxicolalines A (1) and N-methylnarceimicine (2), together with sixteen known alkaloids (3 - 18) were isolated from Corydalis saxicola Bunting. The structures of saxicolalines A (1) and N-methylnarceimicine (2) were established based on spectral methods including 1D, 2D NMR (COSY, HMQC, HMBC) and HR-ESI-MS. The anti-HBV activities of ten main alkaloids were evaluated. Among the tested compounds, dihydrochelerythrine (8) exhibited the most potent activity against HBsAg and HBeAg secretions with IC50 < 0.05 μM, SI > 3.5, respectively.
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