Planta Med 2007; 73(8): 769-773
DOI: 10.1055/s-2007-981553
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Allose Gallates Suppress Expression of Pro-Inflammatory Cytokines through Attenuation of NF-κB in Human Mast Cells

Seung Ho Lee1 [*] , Hyo-Hyun Park2 [*] , Jung-Eun Kim3 , Jeong-Ah Kim1 , Yeo Hyang Kim4 , Chang-Duk Jun5 , Sang-Hyun Kim2
  • 1College of Pharmacy, Yeungnam University, Kyongsan, Republic of Korea
  • 2Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
  • 3Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
  • 4Department of Pediatrics, Keimyung University School of Medicine, Daegu, Republic of Korea
  • 5Department of Life Science, Gwangju Institutive of Science and Technology, Gwangju, Republic of Korea
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Publikationsverlauf

Received: January 16, 2007 Revised: May 14, 2007

Accepted: May 14, 2007

Publikationsdatum:
28. Juni 2007 (online)

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Abstract

Gallotannins are plant-derived, water-soluble polyphenols with wide-ranging biological activities. Mast cell-mediated allergic inflammation is known to cause many diseases such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 with immune regulatory properties. Expression of inflammatory cytokines is mainly regulated by a transcription factor, nuclear factor (NF)-κB. In the present study, the effect of eight gallotannins on the level of pro-inflammatory cytokines and NF-κB activation was investigated in human mast cell line (HMC-1). HMC-1 cells were sensitized by phorbol 12-myristate 13-acetate (PMA) and calcium ionophore (A23187). Among the eight gallotannins from Euphorbia species, three gallotannins such as 1,2,3,4,6-penta-O-galloyl-β-D-glucose, 1,2,6-tri-O-galloyl-β-D-allopyanose, and 1,2,3,6-tetra-O-galloyl-β-D-allopyranose suppressed the gene expression and secretion of pro-inflammatory cytokines in a dose-dependent manner. In addition, these three gallotannins blocked the activation of NF-κB as indicated by an NF-κB-dependent gene reporter assay. We conclude that these gallotannins may have potential for the treatment of inflammatory diseases through the down-regulation of NF-κB-mediated activation of mast cells.

Abbreviations

HMC-1:human mast cell

IL:interleukin

NF-κB:nuclear factor-κB

PMA:phorbol 12-myristate 13-acetate

PMACI:PMA and calcium ionophore A23187

References

1 These authors contributed equally to this work

Prof. Sang-Hyun Kim

Department of Pharmacology

School of Medicine

Kyungpook National University

Daegu 700-422

Republic of Korea

Telefon: +82-53-420-4838

Fax: +82-53-423-4838

eMail: shkim72@knu.ac.kr