Vernonia nigritiana Oliv. & Hiern. (Asteraceae) is a widely distributed plant of West Africa where is
traditionally used against dermatoses, digestive insufficiency, fever, rheumatism
and headache [1]. Previous studies revealed a topical anti-inflammatory activity of
a chloroform extract from the aerial parts of V. nigritiana, containing stigmastane-type steroids [2]. Continuing the investigation of V. nigritiana constituents, nine polyhydroxylated stigmasterol glycosides (1-9) and six (10-15) polyhydroxylated stigmastanes having a Δ7(8), 9(11), 24(28) -steroid cyclic system, were isolated. Their structure was elucidated by NMR, 1D-TOCSY,
2D-HOHAHA, COSY-DQF, HSQC, HMBC, 1D-ROESY and 1D-NOESY. The molecular formula of the
compounds was confirmed by MS analysis.
All the compounds were screened for their topical anti-inflammatory activity as inhibition
of the Croton oil-induced ear oedema in mice [3]. Each compound provoked a significant
oedema reduction, the most active being compounds 1 and 12 (ID50=0.10 and 0.21µmol/cm2, respectively). Their effect was only two and five fold lower than that of the steroidal
drug hydrocortisone (ID50=0.04µmol/cm2). Compounds 2-11 and 13-15 provoked inhibitions in the range of 31–83% at the highest dose (0.5µmol/cm2). As reference, the parent compounds stigmasterol and stigmastanol (0.5µmol/cm2) were inactive. To clarify a possible mechanism of action, these compounds were evaluated
for their ability to inhibit NF-κB, a transcription factor regulating the expression
of inflammatory macromolecules. Studies, carried out in an electrophoretic mobility
shift assay [4], showed a complete NF-κB inhibition induced by compounds 1 and 5 (50µM), and a slight inhibition for compounds 6 (50µM) and 8 (20–100µM).
References: [1] Igile, G. et al. (1995) J. Nat. Prod. 58: 1438–1443. [2] Cioffi G. et al. (2001)
International Symposium of the Phytochemical Society of Europe. Lausanne, 12th-14th September 2001. [3] Tubaro A. et al. (1985) Agents Actions 17: 317–319. [4] Lyss
G. et al. (1997) Biol. Chem. 378: 951–961.
