Antioxidant activities and DNA fingerprint of 4 varieties Lotus stamens

N. Phonkot; P. P. Wangsomnuk; C. Aromdee

doi:10.1055/s-2007-987029

Planta Medica, Inhaltsverzeichnis

Antioxidant activities and DNA fingerprint of 4 varieties Lotus stamens

N Phonkot ¹, PP Wangsomnuk ², C Aromdee ³

¹Graduate student, Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002, Thailand
²Department of Biology, Faculty of Science, Khon Kaen University, 40002, Thailand
³Corresponding author, Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002, Thailand

Abstract

Stamens of sacred lotus (Nelumbo nucifera Gaerth), or Royal Lotus in Thai, are used as a heart stimulant and as an active ingredient in many Thai traditional recipes. This work is part of the standard and specification establishment of lotus stamens used in Thai traditional medicine [1]. Stamens from four varieties of Nelumbo nucifera (Gaerth) were investigated for their DNA fingerprints, and their antioxidant activities, DPPH and TBARS [2–6]. The PCR amplification was used for identification of Lotus DNA by using OPS3, OPS11, OPS13 and OPE3 random-decamer primers. The result showed variety-specific markers of Pathum, Sattabongkot, Boontharik and Sattabutre varieties. The antioxidant activities (IC₅₀) of these four Lotus varieties by 2,2-diphenyl-1-picrylhydrazyl (DPPH) model were 69.0±6.3, 62.5±4.0, 32.3±3.4 and 40.5±1.5µg.mL^-1, respectively. The thiobarbituric acid reactive substance (TBARS) model were 47.3±6.3, 45.3±2.3, 23.0±5.6 and 29.1±2.3µg.mL^-1, respectively. The results showed that IC₅₀ in both methods of Sattabongkot were significantly low (p<0.05, at the confidence level of 95%).

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