Planta Med 2007; 73 - P_288
DOI: 10.1055/s-2007-987068

Pressurized liquid extraction of tectoridin and tectorigenin in Belamcanda chinensis and pressurized water extraction of phyllodulcin in Hydrangea macrophylla

HJ Lee 1, BH Um 1, KD Yoon 2, J Kim 2, CY Kim 1
  • 1KIST Gangneung Institute, Gangneung Techno Valley, Gangneung, Gangwon-do 210–340, Korea
  • 2College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151–742, Korea

Pressurized liquid extraction (PLE) was applied to extract tectoridin and tectorigenin from Belamcanda chinensis (Iridaceae) with aqueous ethanol, an environment-friendly solvent. Tectoridin and tectorigenin were proven to be the main biologically active constituents of B. chinensis with anti-inflammatory, anti-angiogenic, and anti-tumor activities [1]. Several compositions of water and ethanol, temperatures, and static extraction times were studied to optimize extraction protocol. The optimized conditions are as follows: (i) tectoridin; solvent, 60% ethanol/water (v/v); temperature, 150 oC; static extraction time, 10min; (ii) tectorigenin; solvent, 60% ethanol/water (v/v); temperature, 180 oC; static extraction time, 15min. In addition, extraction efficiency of PLE for tectoridin and tectorigenin was compared to conventional methods such as soxhlet and sonication extraction. The highest extraction efficiency of tectoridin and tectorigenin was achieved by PLE. Pressurized hot water extraction (PHWE) was developed for phyllodulcin, a well known sweetener, in Hydrangea macrophylla var. thunbergii Makino (Saxifragaceae) [2]. PHWE is a non-flammable, harmless, readily available and eco-friendly extraction method. The extraction yield was highest at 150 oC and 20min. In addition, extraction efficiency of PHWE for phyllodulcin was compared to sonication extraction with methanol. The extraction yields of phyllodulcin were 10.4mg/g for PHWE, and 17.4mg/g for sonication with methanol. From these results, it is possible to state that PLE and PHWE could be promising alternative extraction methods for natural products.

Acknowledgements: No. RT105–01–02 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (Republic of Korea), Gangneung Marine Bio Foundation (Korea).

References: [1] Jung, S. H. et al. (2003) Planta Med. 69: 617–622. [2] Yasuda, T. et al. (2004) J. Nat. Prod. 67: 1604–1607.