Infusions of aerial parts of Ivan-tea (Chamaenerion angustifolium, Onagraceae) and Kurilian tea (Pentaphylloides fruticosa, Rosaceae) have been used in Russia for a long time as home made teas. The data about the chemical composition of these plants are limited. Dry water-soluble extracts of C. angustifolium (5 series) and P. fruticosa were investigated. The botanical material was dried at 40°C for 5–6h. After comminution, the tea was placed in heaps on outside platforms, wetted and bruised to initiate „fermentation“, and fermented for 6h at 40–41°C (except the unfermented sample). Fermented teas were sampled from the heaps and dried at 40°C for 4–6h. The material obtained was suspended in water and extracted for 2h. The resulting aqueous extracts were filtered, reduced in volume in vacuo, dried, and stored at 4°C. The extracts were analyzed by RP HPLC coupled with PDA detection. Results of the analysis of the extracts are presented in table below.
Table 1. Qualitative-quantitative data for a water soluble extracts of C. angustifolium and P. fruticosa
|
|
|
Identified components, mg/g
|
|
Extract
|
Total
phenols
|
L-asc. acid
|
Gallic acid
|
Protocatechuic acid
|
Hyperoside
|
Ellagic acid
|
Octyl
gallate
|
|
C. angustifolium
|
|
|
|
|
|
|
|
|
Express
|
194.4±7.9
|
ND
|
2.08±0.10
|
2.88±0.14
|
2.93±0.14
|
0.96±0.05
|
0.12±0.01
|
|
Lotos
|
145.5±14.2
|
ND
|
1.07±0.05
|
3.22±0.15
|
3.00±0.14
|
0.59±0.02
|
0.15±0.01
|
|
Tver
|
206.9±19.4
|
15.9±0.8
|
4.70±0.18
|
ND
|
2.82±0.10
|
1.07±0.06
|
0.08±0.01
|
|
Diod
|
156.8±11.4
|
17.7±0.9
|
4.46±0.16
|
ND
|
3.53±0.15
|
1.18±0.06
|
0.08±0.01
|
|
Nonfermented
|
209.4±4.2
|
6.1±0.1
|
4.47±0.24
|
ND
|
2.92±0.14
|
1.06±0.05
|
0.09±0.01
|
|
P. fruticosa
|
160.6±14.5
|
9.4±0.4
|
4.04±0.20
|
ND
|
3.08±0.14
|
1.01±0.03
|
0.08±0.01
|
All the extracts of C. angustifolium contained gallic acid, hyperoside, ellagic acid, and octyl gallate. L-ascorbic acid was identified only in Tver, Diod and nonfermented extracts. Protocatechuic acid was identified only in Express and Lotos extracts. Extract of P. fruticosa contained L-ascorbic acid, gallic acid, hyperoside, ellagic acid and octyl gallate. Protocatechuic acid was not detected in this extract. It can be seen that the water-soluble extracts of C. angustifolium and P. fruticosa in general have similar chromatographic profiles and active components in equivalent concentration.
