Planta Med 2007; 73 - P_302
DOI: 10.1055/s-2007-987082

HPLC evaluation of water-soluble extracts of Chamaenerion angustifolium L. and Pentaphylloides fruticosa L

A Shikov 1, E Poltanov 2, O Pozharitskaya 2, HJD Dorman 3, V Makarov 1, V Tikhonov 2, R Hiltunen 3
  • 1Interregional Center “Adaptogen“, 47/5, Piskarevsky pr., 195067, St-Petersburg, Russia
  • 2“Diod“ Ltd, 11
  • aDerbenevskaya ave., 113114, Moscow, Russia
  • 3Faculty of Pharmacy, Div. of Pharm. Biology, University of Helsinki, P.O. Box 56 (Viikinkaari 5E), FIN-00014, Finland

Infusions of aerial parts of Ivan-tea (Chamaenerion angustifolium, Onagraceae) and Kurilian tea (Pentaphylloides fruticosa, Rosaceae) have been used in Russia for a long time as home made teas. The data about the chemical composition of these plants are limited. Dry water-soluble extracts of C. angustifolium (5 series) and P. fruticosa were investigated. The botanical material was dried at 40°C for 5–6h. After comminution, the tea was placed in heaps on outside platforms, wetted and bruised to initiate „fermentation“, and fermented for 6h at 40–41°C (except the unfermented sample). Fermented teas were sampled from the heaps and dried at 40°C for 4–6h. The material obtained was suspended in water and extracted for 2h. The resulting aqueous extracts were filtered, reduced in volume in vacuo, dried, and stored at 4°C. The extracts were analyzed by RP HPLC coupled with PDA detection. Results of the analysis of the extracts are presented in table below.

Table 1. Qualitative-quantitative data for a water soluble extracts of C. angustifolium and P. fruticosa

Identified components, mg/g

Extract

Total
phenols

L-asc. acid

Gallic acid

Protocatechuic acid

Hyperoside

Ellagic acid

Octyl
gallate

C. angustifolium

Express

194.4±7.9

ND

2.08±0.10

2.88±0.14

2.93±0.14

0.96±0.05

0.12±0.01

Lotos

145.5±14.2

ND

1.07±0.05

3.22±0.15

3.00±0.14

0.59±0.02

0.15±0.01

Tver

206.9±19.4

15.9±0.8

4.70±0.18

ND

2.82±0.10

1.07±0.06

0.08±0.01

Diod

156.8±11.4

17.7±0.9

4.46±0.16

ND

3.53±0.15

1.18±0.06

0.08±0.01

Nonfermented

209.4±4.2

6.1±0.1

4.47±0.24

ND

2.92±0.14

1.06±0.05

0.09±0.01

P. fruticosa

160.6±14.5

9.4±0.4

4.04±0.20

ND

3.08±0.14

1.01±0.03

0.08±0.01

All the extracts of C. angustifolium contained gallic acid, hyperoside, ellagic acid, and octyl gallate. L-ascorbic acid was identified only in Tver, Diod and nonfermented extracts. Protocatechuic acid was identified only in Express and Lotos extracts. Extract of P. fruticosa contained L-ascorbic acid, gallic acid, hyperoside, ellagic acid and octyl gallate. Protocatechuic acid was not detected in this extract. It can be seen that the water-soluble extracts of C. angustifolium and P. fruticosa in general have similar chromatographic profiles and active components in equivalent concentration.