Induced proliferation arrest of a non-small cell lung carcinoma cell line (NSCLC-N6) by a new 4-phenylcoumarin

In spite of our growing insight into carcinogenesis, treatment of tumors, especially non-small cell lung cancer (NSCLC), remains limited and it is urgent to develop strategies that target tumor cells and their genetic features. In this regard, our work was concerned with genetic modifications arising in an in vitro NSCLC cell line after treatment with the newly synthesized 4-phenylcoumarin Mammea A/BA deshydrocyclo F, regioisomer of Mammea A/AA deshydrocyclo F isolated from the aerial part of the tropical plant Calophyllum dispar. First, it has been shown that Mammea A/BA deshydrocyclo F induces arrest of proliferation by blocking cells in G1 phase of the cell cycle. Then „differential display“ strategy has been used to clarify the genetic mechanisms involved in this arrest of proliferation. Therefore, these data provide new insights about mechanisms participating in arrest of proliferation of tumour cells.