Planta Med 2007; 73(14): 1481-1485
DOI: 10.1055/s-2007-990249
Pharmacology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Inhibition of HCV Replicon Cell Growth by 2-Arylbenzofuran Derivatives Isolated from Mori Cortex Radicis

Hyun Yong Lee1 [*] , Ji Hyun Yum1 [*] , Yang Kook Rho1 , Seong Jun Oh1 , Hyun Sik Choi1 , Hwan Bong Chang1 , Dong Hwa Choi1 , Moon-Jeong Leem1 , Eun Joo Choi1 , Jei Man Ryu1 , Soon Bong Hwang2
  • 1R&D Center, Dong-Wha Pharmaceutical Ind. Co. Ltd., Anyang, Korea
  • 2Ilsung Institute of Life Science, Hallym University, Chuncheon, Korea
Further Information

Publication History

Received: May 4, 2007 Revised: September 11, 2007

Accepted: September 16, 2009

Publication Date:
18 October 2007 (online)

Abstract

Medicinal herbs are increasingly used in the search for safe and efficient drug candidates for hepatitis C virus infection. In this study, we have investigated the anti-HCV effect of compounds from Mori Cortex Radicis. During a screening for extracts with anti-HCV affinity from medicinal plants (173 species), the methanol extract of Mori Cortex Radicis was selected. Fractionation of the extract by monitoring antiviral activity with a replicon cell-based assay resulted in the isolation of five compounds, mulberroside C (1), moracin P (2), moracin O (3), moracin M (4) and mulberrofuran K (5) from the ethyl acetate-soluble fraction. Compounds 14 showed significant inhibitory activities. Compounds 2 and 3 showed potent inhibitory activity (IC50 35.6 μM, 80.8 μM, respectively) in the replicon cell assay, which was confirmed by NS3 helicase inhibitory activity (IC50 42.9 μM, 27.0 μM, respectively).

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1 These authors coordinated equally with this paper

Moon Jeong Leem, PhD

Natural Product Lab, R&D Center

Dong-Wha Pharmaceutical Ind. Co., Ltd.

189 Anyang 7-Dong

Anyang City

Kyonggi-Do

Korea

Phone: +82-31-445-2485

Fax: +82-31-446-9556

Email: natl@iris.dwcrc.co.kr