A simple, rapid and cost-saving method for the determination of total anthocyanins
in roselle has been developed. The method was based on pH-differential spectrophotometry.
The calibration curve of the major anthocyanin in roselle, delphinidin 3-sambubioside
(Dp-3-sam), was constructed by using methyl orange and their correlation factor. The
reliability of this developed method was comparable to the direct method using standard
Dp-3-sam and the HPLC method. Quality characteristics of roselle produced in Thailand
were also reported. Its physical quality met the required specifications. The overall
chemical quality was herein surveyed for the first time and it was found to be the
important parameter corresponded to the commercial grading of roselle. Total contents
of anthocyanins and phenolics were proportional to the antiradical capacity.
Hibiscus sabdariffa
- roselle - Malvaceae - anthocyanins - pH-differential spectrophotometry - method
development - validation - quality