Comparison of Rapid Tests for Detection of Group B Streptococcal Colonization

Cheryl K. Walker; William R. Crombleholme; Marilyn J. Ohm-Smith; Richard L. Sweet

doi:10.1055/s-2007-999247

American Journal of Perinatology, Inhaltsverzeichnis

Am J Perinatol 1992; 9(4): 304-308
DOI: 10.1055/s-2007-999247

ORIGINAL ARTICLE

Comparison of Rapid Tests for Detection of Group B Streptococcal Colonization

Cheryl K. Walker, William R. Crombleholme, Marilyn J. Ohm-Smith, Richard L. Sweet

Departments of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco General Hospital, San Francisco, California

Abstract

ABSTRACT

Accurate rapid detection of maternal lower genital tract colonization with group B streptococci (GBS) in high-risk patients is essential for selective institution of intrapartum antibiotic treatment to reduce neonatal GBS infection. In this study, pure GBS isolates were used to evaluate five commercially available rapid tests in terms of speed, ease of use, and sensitivity. The products tested were Directigen, Equate, Bactigen, PathoDx, and Phadebact. Although each test could be performed relatively quickly, the ease of performance and level of sensitivity (10⁵ to 10⁸ CFU/ml) varied markedly. Quantitative cultures obtained from 17 known GBS carriers showed concentrations ranging from <10² to >10⁸ CFU/gm of vaginal material. Since only 40% of the women had ≥10⁵ CFU/gm of vaginal material, it appears that many colonized women would not be identified by these rapid tests.

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