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DOI: 10.1055/s-2008-1034276
© Georg Thieme Verlag KG Stuttgart · New York
Tissue Specificity and Developmental Pattern of Amorpha-4,11-diene Synthase (ADS) Proved by ADS Promoter-Driven GUS Expression in the Heterologous Plant, Arabidopsis thaliana
Publikationsverlauf
Received: April 12, 2007
Revised: October 9, 2007
Accepted: December 4, 2007
Publikationsdatum:
17. Januar 2008 (online)
![](https://www.thieme-connect.de/media/plantamedica/200802/lookinside/thumbnails/10.1055-s-2008-1034276-1.jpg)
Abstract
Amorpha-4,11-diene synthase (ADS) of Artemisia annua L. is a sesquiterpene cyclase that catalyzes the conversion of farnesyl diphosphate into amorpha-4,11-diene in the biosynthesis of the antimalarial artemisinin. To explore the mechanisms regulating the tissue-specific and developmental distributions of ADS, a full ADS promoter was generated using PCR, and fused to GUS for introduction into Arabidopsis thaliana. ADSpro::GUS fusion transcripts were organ-specific, mainly present in the anthers and trichomes of the green tissues of the juvenile leaves. This result was consistent with the ADS transcription pattern observed in A. annua as examined by RT-PCR. To determine the subcellular localization of ADS, an open reading frame (ORF) of ADS was fused to the green fluorescent protein (smGFP) gene and introduced into the A. thaliana protoplasts. GFP fluorescence was located exclusively in the cytosol, an indication that ADS is a cytosol-localized protein.
Abbreviations
ADS:amorpha-4,11-diene synthase
ADSpro:amorpha-4,11-diene synthase promoter
MS medium:Murashige and Skoog medium
ORF:open reading frame
PCR:polymerase chain reaction
RACE:rapid amplification of cDNA ends
PLM-RACE:RNA ligase mediated RACE
RT-PCR:reverse transcriptase-PCR
TAIL-PCR:thermal asymmetric interlaced-PCR
Key words
Artemisia annua - Arabidopsis thaliana - Asteraceae - amorpha-4,11-diene synthase (ADS) - amorpha-4,11-diene synthase promoter (ADSpro)
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Prof. Soo-Un Kim
Program in Applied Life Chemistry
Department of Agricultural Biotechnology
Seoul National University
Seoul 151-921
Korea
Telefon: +82-2-880-4642
Fax: +82-2-873-3112
eMail: soounkim@plaza.snu.ac.kr