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DOI: 10.1055/s-2008-1034279
© Georg Thieme Verlag KG Stuttgart · New York
Genetic Characterization and Authentication of Embelia ribes using RAPD-PCR and SCAR Marker
Publikationsverlauf
August 11, 2007
December 9, 2007
Publikationsdatum:
21. Januar 2008 (online)
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Abstract
Embelia ribes Burm. f. (Myrsinaceae) is one of the important plants used in Indian traditional medicine. RAPD-PCR analysis was performed to obtain species-specific DNA fragments. A band of 906 bp that was specific to Embelia ribes irrespective of the geographical source was obtained using the random decamer primer OPF 05. SCAR primers ER 1 (27 mer) and ER 2 (26 mer) were designed from the sequence of the RAPD marker which yielded an expected amplicon of 594 bp with the Embelia ribes DNA only. This methodology can be used for species identification of genuine Embelia ribes and to distinguish it from common substitutes and adulterants.
Abbreviations
BLAST: basic local alignment search tool
ER 1: Embelia ribes forward primer
ER 2: Embelia ribes reverse primer
RAPD-PCR: random amplification of polymorphic DNA polymerase chain reaction
SCAR: sequence characterized amplified region
Key words
Embelia ribes Burm. f. - Myrsinaceae - adulteration - RAPD-PCR - SCAR - authentication
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Dr. Padma Venkatatasubramanian
Centre for Pharmacognosy & Product Development of Traditional Medicines
Foundation for Revitalization of Local Health Traditions (FRLHT)
74/2, Jarakabande Kaval
PO Attur Via Yelahanka
Bangalore-560064
India
Telefon: +91-80-28565618
Fax: +91-80-28567926
eMail: padma.venkat@frlht.org