Abstract
The effects of four fractions, a hexane fraction, an ethyl acetate fraction, an n-butanol fraction and a water fraction, from a water extract of Herba Visci were investigated to find the fraction, in in vitro experiments, with the greatest ability to inhibit the formation of osteoclast-like multinucleated cells from mouse bone marrow cells and also to inhibit 45Ca release from a mouse parietal bone organ culture system. The ethyl acetate fraction was able to inhibit the formation of osteoclast-like cells in a dose-dependent manner and was also able to potently inhibit the release of 45Ca even at a low concentration. Therefore, a further in vivo experiment was performed to determine if the ethyl acetate fraction had antiosteoporotic activity. It was found to inhibit the decreases in total and cancellous bone mineral density, in cancellous and cortical bone mineral content as well as in cortical bone thickness and in the X-axis strength index of tibiae of ovariectomized rats. The principle constituents of the ethyl acetate fraction were determined to be (+)-syringaresinol O-β-glucopyranoside, 2-homoeriodictyol 7-O-glucoside and viscumneoside I by HPLC analysis.
Abbreviations
BMC:bone mineral content
BMD:bone mineral density
BuOH:n-butanol fraction
BuOH-44:44 μg/mL n-butanol fraction
BuOH-440:440 μg/mL n-butanol fraction
EtOAc:ethylacetate fraction
EtOAc-44:44 μg/mL ethylacetate fraction
EtOAc-440:440 μg/mL ethylacetate fraction
Extract-44:44 μg/mL water extract
Extract-440:440 μg/mL water extract
FBS:fetal bovine serum
Hexane-44:44 μg/mL hexane fraction
Hexane-440:440 μg/mL hexane fraction
α-MEM:alphy modified Eagle's medium
pQCT:peripheral quantitative computed tomography
PSSI:polar-axis strength index
PTH:parathyroid hormone
Water-44:44 μg/mL water fraction
Water-440:440 μg/mL water fraction
XSSI:X-axis strength index
YSSI:Y-axis strength index
Key words
Viscum coloratum
- Loranthaceae - osteoclast - bone resorption - ovariectomy - pQCT
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Prof. Jun Yin
Department of Pharmacognosy
Shenyang Pharmaceutical University
103 Wenhua Road
Shenyang 110016
People’s Republic of China
Telefon: +86-24-2398-6491
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