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DOI: 10.1055/s-2008-1034289
© Georg Thieme Verlag KG Stuttgart · New York
Identification of Medicinal Ganoderma Species Based on PCR with Specific Primers and PCR-RFLP
Publication History
Received: August 19, 2007
Revised: November 29, 2007
Accepted: December 14, 2007
Publication Date:
04 February 2008 (online)
![](https://www.thieme-connect.de/media/plantamedica/200802/lookinside/thumbnails/10.1055-s-2008-1034289-1.jpg)
Abstract
Ganoderma lucidum is an interesting shelf fungus that is also of medical importance in East Asian countries such as China, Japan and Korea. The fruit bodies of different Ganoderma species have similar colors and shapes, but are different regarding their medicinal properties. In order to provide molecular evidence for the genuine origin of G. lucidum, G. sinense and G. tsuga, fungal immunomodulatory protein (FIP) genes were cloned using genomic walker technology and their sequences were then determined. Based on the sequences of cloned FIP genes, we designed three pairs of species-specific primers for amplification and sequencing of FIP genes. These new primers allowed an effective amplification and sequencing of FIP genes from representative species of G. lucidum, G. sinense and G. tsuga. Phylogenetic relationships were resolved quite efficiently by the FIP gene sequences, which proved to be more useful than other genetic marker techniques in phylogenetic reconstruction of G. lucidum, G. sinense and G. tsuga.
Key words
Ganoderma - fungal immunomodulatory protein - identification - PCR
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Prof. Xuanwei Zhou
Plant Biotechnology Research Center
Fudan-SJTU-Nottingham Plant Biotechnology R&D Center
School of Agriculture and Biology
Shanghai Jiao Tong University
Huashan Road 1954
Shanghai 200030
People’s Republic of China
Phone: +86-21-6564-2425
Fax: +86-21-6564-2425
Email: xuanweizhou@sjtu.edu.cn
Prof. Juan Lin, Ph.D.
State Key Laboratory of Genetic Engineering
School of Life Sciences
Morgan-Tan International Center for Life Sciences
Fudan-SJTU-Nottingham Plant Biotechnology R&D Center
Fudan University
220 Handan Road
Shanghai 200433
People’s Republic of China
Phone: +86-21-6564-2772
Fax: +86-21-6564-3552
Email: linjuan@fudan.edu.cn