Differentiation of the Traditional Chinese Medicinal Plants Euphorbia humifusa and E. maculata from Adulterants by TaqMan Real-Time Polymerase Chain Reaction

Abstract

DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reaction were exploited for their applications in the differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from three related adulterants E. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. humifusa and E. maculata from the adulterants by TaqMan real-time PCR.

Abbreviations

ITS: internal transcribed spacer

Traditional Chinese medicinal plants - Euphorbia humifusa - Euphorbia maculata - Euphorbiaceae - TaqMan real-time polymerase chain reaction

References

• 1 The state Pharmacopoeia Commission of the PRC. Pharmacopoeia of the People’s Republic of China. Beijing; Chemical Industry Press 2005
• 2 Chu X, Fan C, Luo Y. Identification of Euphorbia humifusa Wind. and E. maculata Raf. from its confused species E. thymifolia L.  J Chin Trad Med. 1994;  19 325-7
• 3 Holland P M, Abramson R D, Watson R, Gelfand D H. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase.  Proc Natl Acad Sci USA. 1991;  88 7276-80
• 4 Brodmann P D, Moor D. Sensitive and semi-quantitative TaqMan real-time polymerase chain reaction system for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed.  Meat Sci. 2003;  65 599-607
• 5 Xue H G, Zhou S D, He X J, Yu Y. Molecular authentication of the traditional Chinese medicinal plant Euphorbia pekinensis.  Planta Med. 2007;  73 91-3
• 6 Xue H G, Zhou S D, He X J, Yu Y. Molecular authentication of the traditional Dai medicinal plant Croton caudatus.  Planta Med. 2007;  73 611-3
• 7 Xue C Y, Li D Z, Lu J M, Yang J B, Liu J Q. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii.  Planta Med. 2006;  72 1223-6