Isolation and Characterization of New Cannabis Constituents from a High Potency Variety

Abstract

Phytochemical investigation of a high potency variety of Cannabis sativa L. resulted in the isolation of six new metabolites, (±)-6,7-trans-epoxycannabigerolic acid (2), (±)-6,7-cis-epoxycannabigerolic acid (3), (±)-6,7-cis-epoxycannabigerol (4), (±)-6,7-trans-epoxycannabigerol (5), 5′-methyl-4-pentylbiphenyl-2,2′,6-triol (7), and 7-methoxycannabispirone (8), along with seven known compounds namely, cannabigerolic acid (1), 5′-methoxycannabigerolic acid (6), cannabispirone (9), β-cannabispiranol (10), dehydrocannabifuran (11), cannflavin B (12) and cannabigerol (13). The antimicrobial as well as the antileishmanial activities were investigated.

Abbreviations

CBG:cannabigerol

CBD:cannabidiol

FID:flame ionization detector

Δ⁹-THC:Δ⁹-tetrahydrocannabinol

VLC:vacuum liquid chromatography

Key words

Cannabis sativa - Cannabaceae - epoxy cannabigerolic acid - cannabispirone - antimicrobial activity - antileishmanial activity

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