Planta Med 2008; 74(5): 503-508
DOI: 10.1055/s-2008-1034373
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

In vivo Efficacy of Calceolarioside A against Experimental Visceral Leishmaniasis

Avijit Poddar1 , Anindita Banerjee1 , Srijani Ghanta1 , Sharmila Chattopadhyay1
  • 1Drug Development/Diagnostics & Biotechnology Division, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata 700 032, India
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Publikationsverlauf

Received: January 9, 2008 Revised: February 14, 2008

Accepted: February 22, 2008

Publikationsdatum:
01. April 2008 (online)

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Abstract

Bioactivity-guided fractionation has led to the successful isolation of calceolarioside A (1) from the methanolic extract of night jasmine leaves. The in vitro antileishmanial activity of compound 1 was determined (IC50 = 20 μg/mL). Its in vivo efficacy was noted at 20 mg/kg body weight when it reduced the hepatic and splenic parasite burden by 79 and 84 %, respectively, in an established model of L. donovani Ag83 infected golden hamster. Furthermore, synergistic potentiations of compound 1 at 20 mg/kg body weight and SAG at 5 mg/kg body weight showed a significant reduction of hepatic and splenic parasite burden. No cytotoxicity was observed against the U937 cell line. This is the first report describing the isolation of compound 1 from N. arbor-tristis L. and the first demonstration of its potent activity against visceral leishmaniasis.

Abbreviations

ABTS:2,2′-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid)

Cal A:calceolarioside A

DMSO:dimethyl sulphoxide

HOLC:high pressure liquid chromatography

K. Glucoside:kaempferol 3-β-D-glucoside

LDU:leishman donovan unit

PE:semi pure bioactive fraction

SAG:sodium antimony gluconate

References

Dr. (Mrs.) Sharmila Chattopadhyay

Drug Development, Diagnostics & Biotechnology Division

Indian Institute of Chemical Biology

4 Raja S. C. Mullick Road

Kolkata 700 032

India

Telefon: +91-033-2473-3491

Fax: +91-033-2473-5197

eMail: sharmila@iicb.res.in