Subscribe to RSS
DOI: 10.1055/s-2008-1066876
Comparison of Two Rapid Strip Tests Based on IGFBP-1 and PAMG-1 for the Detection of Amniotic Fluid
Publication History
Publication Date:
25 March 2008 (online)
ABSTRACT
Our objective was to compare two rapid strip tests for the detection of amniotic fluid, based on the detection of insulin-like growth factor-binding protein-1 (IGFBP-1) and of placental α-microglobulin-1 (PAMG-1). Samples of amniotic fluid were taken in 20 pregnant women between 31 3/7 and 41 2/7 gestational weeks at elective cesarean section before delivery of the newborn. These samples were diluted with 0.9% saline solution in a dilution series down to concentrations of 1:320. Immunoassay strip tests were then compared in their ability to detect remaining concentrations of amniotic fluid. In 5 cases, both test methods showed the same results. In all remaining 15 cases, the test based on PAMG-1 proved to be superior by detecting amniotic fluid at least at one descending concentration below the test based on IGFBP-1. Thus, the rapid strip test based on PAMG-1 seems to be a more sensitive bedside test compared with the test based on IGFBP-1 for the detection of amniotic fluid.
KEYWORDS
Amniotic fluid - bedside test - insulin-like growth factor-binding protein-1 - placental α-microglobulin-1
REFERENCES
- 1 Lockwood C J, Wein R, Chien D, Ghidini A, Alvarez M, Berkowitz R L. Fetal membrane rupture is associated with the presence of insulin-like growth factor-binding protein-1 in vaginal secretion. Am J Obstet Gynecol. 1994; 171 146-150
- 2 Rutanen E M, Karkkainen T H, Lehtovirta J, Uotila J T, Hinkula M K, Hartikainen A L. Evaluation of a rapid strip test for insulin-like growth factor binding protein-1 in the diagnosis of ruptured fetal membranes. Clin Chim Acta. 1996; 253 91-101
- 3 Darj E, Lyrenäs S. Insulin-like growth factor binding protein-1, a quick way to detect amniotic fluid. Acta Obstet Gynecol Scand. 1998; 77 295-297
- 4 Guibourdenche J, Luton D, Andre E, Noel M, Porquet D. Rapid detection of insulin-like growth factor-binding protein-1 and foetal fibronectin in cervico-vaginal secretions to diagnose premature membrane rupture. Ann Clin Biochem. 1999; 36 388-390
- 5 Wathen N C, Egembah S, Campbell D J, Farkas A, Chard T. Levels of insulin-like growth factor-binding protein-1 increase rapidly in amniotic fluid from 11 to 16 weeks of pregnancy. J Endocrinol. 1993; 137 R1-R4
- 6 Rutanen E M, Pekonen F, Karkkainen T. Measurement of insulin-like growth factor binding protein-1 in cervical/vaginal secretions: comparison with the ROM-check Membrane Immunoassay in the diagnosis of ruptured fetal membranes. Clin Chim Acta. 1993; 214 73-81
- 7 Petrunin D D, Griaznova I M, Petrunina IuA, Tatarinov IuS. Immunochemical identification of organ specific human placental alpha-globulin and its concentration in amniotic fluid. Akush Ginekol (Mosk). 1977; (1) 62-64
- 8 Cousins L M, Smok D P, Lovett S M, Poeltler D M. AmniSure placental alpha macroglobulin-1 rapid immunoassay versus standard diagnostic methods for detection of rupture of membranes. Am J Perinatol. 2005; 22 317-320
- 9 Lee S E, Park J S, Norwitz E R, Kim K W, Park H S, Jun J K. Measurement of placental alpha-microglobulin-1 in cervicovaginal discharge to diagnose rupture of membranes. Obstet Gynecol. 2007; 109 634-640
Dr. med. Frank Chih-Kang Chen
Klinik fuer Geburtsmedizin, Charité Universitaetsmedizin Berlin
Campus Virchow-Klinikum, Augustenburger Platz 1,13353 Berlin, Germany