Planta Med 2008; 74(7): 719-725
DOI: 10.1055/s-2008-1074526
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

HPLC-MS Analysis and Anti-Oedematogenic Activity of Hypericum grandifolium Choisy (Hypericaceae)

Celia X. Bonkanka1 , Andrija Smelcerovic2 , Sebastian Zuehlke2 , Rosa María Rabanal1 , Michael Spiteller2 , Candelaria del Carmen Sánchez-Mateo1
  • 1Department of Pharmacology, Faculty of Pharmacy, University of La Laguna, La Laguna, Tenerife, Spain
  • 2Institute of Environmental Research, University of Dortmund, Dortmund, Germany
Weitere Informationen

Publikationsverlauf

Received: September 25, 2007 Revised: March 11, 2008

Accepted: March 15, 2008

Publikationsdatum:
16. Mai 2008 (online)

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Abstract

A study has been carried out on the chemical composition and in vivo anti-oedematogenic activity of several extracts from Hypericum grandifolium Choisy (Hypericaceae) collected in Tenerife (Canary Islands). The HPLC-MS analysis (HPLC-ESI/MS and HPLC-FT/MS) revealed the presence of hyperforin, flavonoids and probably benzophenone derivatives, while naphthodianthrones were absent. Pharmacological results revealed that the methanol extracts and the aqueous, butanol and chloroform fractions obtained therefrom possess anti-oedematogenic activity against TPA-induced ear oedema and carrageenan-induced paw oedema, the chloroform fraction being the most active. Subfractions derived from the chloroform fraction also showed anti-oedematogenic activity. From these results, it can be suggested that different constituents, such as the flavonoids and the benzophenone derivatives, could be responsible, at least in part, for the anti-oedematogenic effects observed for this species.

References

Prof. Dr. Candelaria del Carmen Sánchez-Mateo

Department of Pharmacology

Faculty of Pharmacy

University of La Laguna

Astrofísico Francisco Sánchez s/n

38071 La Laguna

Tenerife

Spain

Telefon: +34-922-318-502

Fax: +34-922-318-514

eMail: csanchez@ull.es