Abstract
A study has been carried out on the chemical composition and in vivo anti-oedematogenic activity of several extracts from Hypericum grandifolium Choisy (Hypericaceae) collected in Tenerife (Canary Islands). The HPLC-MS analysis (HPLC-ESI/MS and HPLC-FT/MS) revealed the presence of hyperforin, flavonoids and probably benzophenone derivatives, while naphthodianthrones were absent. Pharmacological results revealed that the methanol extracts and the aqueous, butanol and chloroform fractions obtained therefrom possess anti-oedematogenic activity against TPA-induced ear oedema and carrageenan-induced paw oedema, the chloroform fraction being the most active. Subfractions derived from the chloroform fraction also showed anti-oedematogenic activity. From these results, it can be suggested that different constituents, such as the flavonoids and the benzophenone derivatives, could be responsible, at least in part, for the anti-oedematogenic effects observed for this species.
Key words
Hypericum grandifolium Choisy - Hypericaceae - phenolic compounds - HPLC-MS/MS - HPLC-FT/MS -
in vivo anti-oedematogenic activity.
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Prof. Dr. Candelaria del Carmen Sánchez-Mateo
Department of Pharmacology
Faculty of Pharmacy
University of La Laguna
Astrofísico Francisco Sánchez s/n
38071 La Laguna
Tenerife
Spain
Phone: +34-922-318-502
Fax: +34-922-318-514
Email: csanchez@ull.es
