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DOI: 10.1055/s-2008-1074537
© Georg Thieme Verlag KG Stuttgart · New York
Authentication of the Traditional Medicinal Plant Eleutherococcus senticosus by DNA and Chemical Analyses
Publikationsverlauf
Received: November 11, 2007
Revised: March 14, 2008
Accepted: March 31, 2008
Publikationsdatum:
26. Mai 2008 (online)
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Abstract
Shigoka (SGK), the rhizome of Eleutherococcus senticosus, is a traditional medicine used as a tonic in northeastern Asia and far eastern Russia. We analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) sequence of the medicine available on the Japanese and Chinese markets and found that at least 3 species were used as the source plant of the commercial SGKs and that only 70 % of all samples was made from the correct species. Furthermore, we performed the quantitative determination of 3 marker compounds, eleutheroside B (EB), syringaresinol diglucoside (Syr), and isofraxidin (Iso) by ultraperformance liquid chromatography (UPLC)/mass spectrometry (MS). We found that EB and Iso are specific to the correct source plant of SGK. Of them, EB is thought to be the best marker compound for quality assurance of the SGK from the viewpoint of its pharmacological activity.
Abbreviations
EB: eleutheroside B
Iso: isofraxidin
ITS: internal transcribed spacer
JP15: Japanese pharmacopoeia 15th edition
MS: mass spectrometry
PCR: polymerase chain reaction
SGK: shigoka
Syr: syringaresinol diglucoside
UPLC: ultraperformance liquid chromatography
Key words
Eleutherococcus senticosus - Araliaceae - ITS sequence - UPLC/MS - eleutheroside B - syringaresinol diglucoside - isofraxidin
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References
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Dr. Yukihiro Goda
National Institute of Health Sciences
Division of Pharmacognosy, Phytochemistry and Narcotics
1-18-1, Kamiyoga, Setagaya-ku
Tokyo 158-8501
Japan
Telefon: +81-3-3700-9154
Fax: +81-3-3707-6950 or +81-3-3700-9165
eMail: goda@nihs.go.jp
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