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Planta Med 2008; 74(10): 1253-1258
DOI: 10.1055/s-2008-1074582
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Effects of Himatanthus lancifolius on Human Leukocyte Chemotaxis and their Adhesion to Integrins

Jeanine Marie Nardin1 , Wesley Maurício de Souza1 , Juliano Ferreira Lopes1 , Ângela Florão1 , Cid Aimbiré de Moraes Santos1 , 2 , Almeriane Maria Weffort-Santos1 , 3
  • 1Pharmaceutical Sciences Post-graduation Program, Federal University of Paraná, Curitiba, Brazil
  • 2Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Paraná, Curitiba, Brazil
  • 3Laboratory of Hematology, Department of Medical Pathology, Federal University of Paraná, Curitiba, Brazil
Further Information

Publication History

Received: December 10, 2007 Revised: May 13, 2008

Accepted: May 14, 2008

Publication Date:
04 July 2008 (online)

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Abstract

The aim of this work was to investigate the anti-inflammatory activities of the uleine-rich fraction extracted from the barks of Himatanthus lancifolius (Muell. Arg.) Woodson (Apocynaceae). To achieve this, we focused on its in vitro effects on some steps of the inflammatory response using peripheral human leukocytes. The results presented herein show that the uleine-rich fraction significantly inhibits the migration of casein-induced granulocytes and their adhesion to fibronectin and vitronectin, along with mononuclear cells, by down-regulating the expression of α4β1 and α5β1 integrins. The data suggest that H. lancifolius has the potential of interferring with leukocyte trafficking through its uleine-rich fraction, emphasizing its usefulness in inflammatory conditions.

Abbreviations

DEXA:dexamethasone disodium phosphate

FN:fibronectin

PMN:polymorphonuclear

URF:uleine-rich fraction

VN:vitronectin

Key words

Himatanthus lancifolius - Apocynaceae - uleine - inflammation - chemotaxis - adhesion molecules

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Prof. Dr. Almeriane Maria Weffort-Santos

Pharmaceutical SciencesPost-graduation Program

Federal University of Paraná

Avenida Prefeito Lothario Meissner 632

80210–070 Curitiba

Brazil

Phone: +55/41/3360/4087

Fax: +55/41/3360/4062

Email: almeriane@ufpr.br