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Planta Med 1980; 38(1): 50-53
DOI: 10.1055/s-2008-1074836
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Spectrofluorometric Determinations of Dihydrofuroquinoline Alkaloids in Choisya ternata Tissue and Cell Cultures

M. Montagu-Bourin, M. Rideau, P. Levillain, J. C. Chénieux
  • Faculté de Pharmacie - 37000 - Tours - France.
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Publication Date:
29 April 2008 (online)

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Abstract

A fluorometric method for the determination of balfourodinium and platy-desminium salts in Choisya ternata H. B. K. cell and tissue cultures is described. These alkaloids possess the same activation wavelength (310 nm) and emission wavelengths at 345 and 448 nm respectively, and are dosed after separation of ion-pair alkaloid-Brom-thymol blue own thin-layer chromatography. A method for a rapid estimation of platydesminium in total extracts is also proposed. This fluorometric method may be extended to other dihydro-furoquinoline alkaloids in Rutaceae extracts (Ptelea, Ruta).

Key Word Index

Choisya ternata - Rutaceae - Alkaloids - Fluorometric Determination.