Abstract
Using glass capillary gas-liquid chromatography and mass spectrometry the alkaloid
patterns of Lupinus polyphyllus LiNDL. plants and cell suspension cultures were analyzed. Sparteine, angustifoline,
lupanine, 13-hydroxylupanine, 17-oxolupanine, 13-cinnamoyllupanine (new for L. polyphyllus), 13-tigloyllupanine and its cis-isomer 13-angeloyllupanine (as a new alkaloid),
and a dehydrolupanine were found in L. polyphyllus leaves and seeds. In addition the presence of other new quinolizidine alkaloids could
be established, too. Cell suspension cultures produce lupanine as the main alkaloid.
The total alkaloid concentration of cell cultures is more than 200 times lower than
that of differentiated lupins, although their biosynthetic capacity to produce alkaloids
is actively expressed as has been shown by precursor feeding experiments and enzymatic
studies.
Key Word Index
Lupinus polyphyllus - Fabaceae (Leguminosae) - Quinolizidine Alkaloids - Cell Suspension
Culture - Capillary GC-MS.
