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Planta Med 1980; 39(6): 112-124
DOI: 10.1055/s-2008-1074912
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Radioimmunoassay for the Determination of the Steroidal Alkaloid Solasodine and Related Compounds in Living Plants and Herbarium Specimens1

E. W. Weiler, H. Krüger, M. H. Zenk
  • Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Bochum, West-Germany
1 Part 13 in the series: „Use of Immunoassay in Plant Sciences”. For part 12 see: E. W. WEILER, Planta (in press).
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Publication Date:
29 April 2008 (online)

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Abstract

A rapid, specific and sensitive radioimmunoassay for the determination of the steroidal alkaloid solasodine is described. The assay allows the detection of as little as 0.7 ng of solasodine glycosides (sola-sonine, solamargine) in crude plant extracts. The detailed distribution of solasodine in vegetative and generative parts of plants of Solanum laciniatum was investigated, and several thousand S. laciniatum plants were screened for individuals with high solasodine content. Plants which contained as much as 3.1 % of solasodine in the leaves were selected for further breeding. A taxonomic survey of the occurrence of solasodine in ca. 250 different species of the genus Solanum using herbarium material is given. 0.5 mg of a herbarium sample is more than sufficient for quantitative analysis.

Key Word Index

Solanum laciniatum - Solarium aviculare - Solanaceae - Solasodine Radioimmunoassay - Taxonomic Distribution of Solasodine.