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Planta Med 1980; 39(6): 148-152
DOI: 10.1055/s-2008-1074917
Research Articles

© Georg Thieme Verlag Stuttgart · New York

The Detection of Helleborus Ecdysteroids by Mass Spectrometry and by GLC Analysis of their TSIM-ethers

Roland Hardman, Tundé V. Benjamin1
  • Pharmacognosy Group, School of Pharmacy and Pharmacology, University of Bath, England
  • 1Present address: Faculty of Pharmacy, University of Ife, Ile-Ife, Nigeria
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Publikationsdatum:
29. April 2008 (online)

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Abstract

20-Hydroxyecdysone (ecdysterone) (2β, 3β, 14α, 20R, 22R, 25-hexahydroxy-5β-cholest-7-en-6-one) and 5β,20-dihydroxyecdysone (Polypodine B) present in extracts of species of Helleborus were detected as their trimethylsilylimidazole (TSIM) derivatives by GLC analysis. The MS analysis of 5β,20-dihydroxyecdysone in the purified extract and the GLC characteristics of the sily-lated ecdysteroids are described and discussed as a means of their identification.

Key Word Index

Helleborus - Ranunculaceae - 20-Hydroxyecdysone - 5β,20-Dihydroxyecdysone.