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Planta Med 1980; 40(9): 55-67
DOI: 10.1055/s-2008-1074942
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Quantitative Bestimmung der Bitterstoffe in Wurzeln von Gentiana lutea und Gentiana purpurea mit HPLC

Quantitative Determination of the Bitter Principles in the Roots of Gentiana lutea and Gentiana purpurea with HPLCO. Sticher, B. Meier
  • Eidgenössische Technische Hochschule Zürich, Pharmazeutisches Institut, Zürich, Schweiz
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Publication History

Publication Date:
29 April 2008 (online)

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Abstract

A HPLC-method for quantitative determination of the bitter substances gentiopicroside, amaropanin, amarogentin and amaroswerin in Gentiana lutea and Gentiana purpurea was developed. A reversed-phase-system with a μBondapak C18 column and various methanol-ater mixtures as eluent were used. The method is adaptable for routine analysis of Gentian extracts. The detection limit for amarogentin was at 233 nm about 10 μg/ ml and for amaropanin 20 μg/ml.

The relative standard deviation is depending on the content of bitter substances. For bitter substance contents > 0.03 % ist was about 3 % and for such < 0.03 % it was about 5 %.

Key Word Index

Gentiana lutea - Gentiana purpurea - Gentianaceae - Bitter Principles - Secoiridoid Glucosides - HPLC - Quantitative Determination.